{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,6]],"date-time":"2026-06-06T03:40:38Z","timestamp":1780717238638,"version":"3.54.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1988,11,1]],"date-time":"1988-11-01T00:00:00Z","timestamp":594345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:p>1. Using a K+\u2010sensitive extracellular electrode, we attempted to determine whether cholinergic stimulation of the simian palm eccrine sweat gland is associated with transient net K+ efflux as in other exocrine glands. 2. When isolated secretory coils placed in a glass capillary were continuously superfused (method A), 32% of total cellular K+ was lost during 3 min of stimulation with methacholine (MCh) followed by K+ reuptake when stimulation was stopped. 3. When secretory coils were stimulated in a small chamber (without continuous superfusion, method C), MCh (5 x 10(\u20106) M)\u2010induced maximal K+ efflux as determined by the peak level of extracellular K+ concentrations was dose dependent, inhibited by atropine but not altered by a cholinesterase inhibitor, physostigmine (1.3 x 10(\u20105) M). Thus the peak K+ level was used as a measure of K+ efflux throughout the study. 4. Phenylephrine (10(\u20104) M) and A23187 (5 x 10(\u20106) M) also induced K+ efflux but to a lesser extent than did MCh. 5. Ouabain (10(\u20103) M)\u2010induced K+ loss was 2.4\u2010fold higher than the peak level of MCh\u2010induced K+ efflux. 6. In a Ca2+\u2010free medium with added EGTA, inhibition of K+ efflux was only partial in the first MCh stimulation but progressively increased on repeated stimulation, suggesting that cytoplasmic or membrane Ca2+ not readily accessible to EGTA may be important for K+ efflux. Inhibition of K+ efflux in the Ca2+\u2010free medium was completely reversed on subsequent addition of Ca2+. 7. Five millimolar Ba2+ partially inhibited MCh\u2010induced K+ efflux. 8. 10(\u20104) M\u2010bumetanide itself caused a small K+ loss and strongly inhibited the subsequent MCh\u2010induced K+ loss. 9. MCh\u2010induced K+ loss was drastically inhibited in the low\u2010Cl\u2010 (by replacing with gluconate\u2010 or methylsulphate\u2010) or low\u2010Na+ (by replacing with Tris+) medium. 10. K+ efflux occurs predominantly across the basolateral membrane. 11. Vinblastine at 10(\u20104) M, which completely inhibits sweat secretion (our unpublished results), however, showed no effect on MCh\u2010induced K+ efflux. 12. We conclude that the transient net K+ efflux associated with MCh stimulation constitutes a crucial primary ionic event in cholinergic eccrine sweat secretion as in other exocrine secretory cells.<\/jats:p>","DOI":"10.1113\/jphysiol.1988.sp017329","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T05:04:13Z","timestamp":1418965453000},"page":"205-217","source":"Crossref","is-referenced-by-count":9,"title":["K+ efflux from the monkey eccrine secretory coil during the transient of stimulation with agonists."],"prefix":"10.1113","volume":"405","author":[{"given":"K","family":"Saga","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1988,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1988.sp017329","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1988.sp017329","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1988.sp017329","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T07:09:21Z","timestamp":1697872161000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1988.sp017329"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,11]]}},"alternative-id":["10.1113\/jphysiol.1988.sp017329"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1988.sp017329","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11]]}}}