{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T07:17:43Z","timestamp":1791011863017,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1988,11,1]],"date-time":"1988-11-01T00:00:00Z","timestamp":594345600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1988,11]]},"abstract":"<jats:p>1. Membrane currents, particularly the outward components, were studied in pineal cells acutely dissociated from adult rats using the whole\u2010cell variant of the patch\u2010clamp technique. 2. In current clamp, outward constant current elicited a transient graded depolarizing response. A sustained membrane rectification developed within 20 ms; this phenomenon was reduced in cells internally dialysed with 120 mM\u2010CsCl. 3. Study of the membrane current revealed the existence of a transient and a delayed outward current. These currents were virtually eliminated when the cell was internally dialysed with CsCl. 4. The delayed outward current, isolated from a holding potential of \u201050 mV, activated at potentials near \u201020 mV, reached a steady\u2010state current amplitude within 60 ms and had little or no decay during steps up to 400 ms in duration. This component was reduced by 80% or more with the addition of 5 mM\u2010TEA. 5. From \u2010100 mV, the transient outward current reached a peak within 15 ms and decayed with a single\u2010exponential time course. The mean decay time constant was 66 +\/\u2010 10 ms (at \u201033 mV) and it showed little voltage sensitivity. This current, which activated at potentials positive to \u201060 mV and displayed half\u2010inactivation at \u201076 +\/\u2010 8 mV, was reduced by 50% with the addition of 5 mM\u20104\u2010AP (4\u2010amino\u2010pyridine). 6. In the presence of external Ca2+, the current\u2010voltage relationship for the delayed current did not display a region of negative\u2010slope conductance (N\u2010shape). Increasing the intracellular ionized Ca2+ concentration by varying the Ca\u2010EGTA buffer ratio did not alter the dependence of the current on the membrane potential. 7. Block of outward currents with internal Cs+ revealed a small (less than 90 pA) inward Ca2+ current when the external Ca2+ concentration was increased to 10 mM. From a holding potential of \u201050 mV, it had a threshold at \u201030 mV and peaked at +5 mV. Evidence for an inward Na+ current was not obtained. 8. We conclude that acutely dissociated pineal cells display two distinct K+ currents: (i) a slowly activating, sustained current similar to the delayed rectifier (IK); and (ii) a transient A\u2010current (IA). At normal Ca2+ concentrations, no macroscopic Ca2+\u2010activated outward current was observed.<\/jats:p>","DOI":"10.1113\/jphysiol.1988.sp017339","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T05:04:13Z","timestamp":1418965453000},"page":"397-419","source":"Crossref","is-referenced-by-count":27,"title":["Characterization of membrane currents in dissociated adult rat pineal cells."],"prefix":"10.1113","volume":"405","author":[{"given":"L G","family":"Aguayo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F F","family":"Weight","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1988,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1988.sp017339","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1988.sp017339","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1988.sp017339","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T07:08:36Z","timestamp":1697872116000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1988.sp017339"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,11]]}},"alternative-id":["10.1113\/jphysiol.1988.sp017339"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1988.sp017339","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,11]]}}}