{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T16:48:01Z","timestamp":1790614081215,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1988,12,1]],"date-time":"1988-12-01T00:00:00Z","timestamp":596937600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1988,12]]},"abstract":"<jats:p>1. Membrane properties and somatostatin effects were studied in cultured locus coeruleus neurones from neonatal rats by using the whole\u2010cell version of the patch clamp technique. 2. The current\u2010voltage relationship of the resting cell revealed an inward\u2010going rectification. The inward currents developed almost instantaneously upon hyperpolarizing the membrane under voltage clamp, and at large negative potentials the inward current showed a time\u2010dependent inactivation. Extracellularly applied Cs+ or Ba2+ (0.1 mM) inhibited the inward current in a voltage\u2010dependent manner. 3. Application of somatostatin (0.01\u20101 microM) produced an increase in membrane conductance. Somatostatin\u2010induced currents were calculated by subtracting the control current from the current during the somatostatin\u2010induced response. The somatostatin\u2010induced current developed almost instantaneously with hyperpolarization and did not show any time\u2010dependent inactivation. The current\u2010voltage relationship of the somatostatin\u2010induced current exhibited a rectification in the inward direction and showed a reversal potential. The reversal potentials were close to the K+ equilibrium potential. 4. Extracellular Cs+ or Ba2+ (0.1 mM) inhibited the somatostatin\u2010induced currents in a voltage\u2010dependent manner, the effectiveness increasing with hyperpolarization. The somatostatin\u2010induced hyperpolarization was not affected by apamin (20 nM) or by charybdotoxin (100 nM). 5. These results indicate that the somatostatin\u2010induced conductance is very similar to the inward\u2010rectification conductance. Because the somatostatin\u2010induced inward rectification did not exhibit a time\u2010dependent inactivation, this rectification and the inward rectification in the control neurones may arise from two different channels. 6. Pre\u2010treatment of neurones with pertussis toxin abolished the somatostatin\u2010induced response, but did not affect the resting inward rectification. When GTP gamma S was applied intracellularly, somatostatin produced an irreversible activation of the inward rectification conductance. The somatostatin\u2010induced hyperpolarization may therefore be mediated through a pertussis toxin\u2010sensitive GTP\u2010binding protein.<\/jats:p>","DOI":"10.1113\/jphysiol.1988.sp017409","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T05:03:53Z","timestamp":1418965433000},"page":"177-198","source":"Crossref","is-referenced-by-count":123,"title":["Somatostatin induces an inward rectification in rat locus coeruleus neurones through a pertussis toxin\u2010sensitive mechanism."],"prefix":"10.1113","volume":"407","author":[{"given":"M","family":"Inoue","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y","family":"Nakajima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1988,12]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1988.sp017409","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1988.sp017409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T06:41:29Z","timestamp":1697956889000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1988.sp017409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1988,12]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1988,12]]}},"alternative-id":["10.1113\/jphysiol.1988.sp017409"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1988.sp017409","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1988,12]]}}}