{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T15:57:47Z","timestamp":1762271867982},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1989,1,1]],"date-time":"1989-01-01T00:00:00Z","timestamp":599616000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1989,1]]},"abstract":"<jats:p>1. Intracellular recordings were made from myenteric neurones isolated from the guinea\u2010pig small intestine to study actions of adenosine 5'\u2010triphosphate (ATP). ATP was applied by superfusion (10 nM\u2010100 microM) or pressure ejection from ATP\u2010containing glass pipettes. 2. Myenteric neurones have been classified into two groups: type I\/S neurones and type II\/AH neurones. ATP produced a membrane hyperpolarization in 80% of AH neurones and a membrane depolarization in 90% of S neurones in a dose\u2010dependent manner. Adenosine caused responses similar to those induced by ATP in both AH and S neurones, but was less effective than ATP. 3. The ATP\u2010induced hyperpolarization was associated with a fall in input resistance, but the ATP\u2010induced depolarization was accompanied by an increase in input resistance. Both responses reversed in polarity near the potassium equilibrium potential (\u201084 to \u201087 mV) and the reversal potential varied with extracellular potassium concentration, as predicted by the Nernst equation. These results indicate that the hyperpolarization is due to an increase, while the depolarization is due to a decrease in potassium conductance. 4. Both the hyperpolarization and the depolarization induced by ATP persisted in calcium\u2010free solution containing 1.2 mM\u2010magnesium, but were markedly reduced or abolished in calcium\u2010free solutions containing 3.7\u201010 mM\u2010magnesium and by 1 mM\u2010nickel or cobalt. Both responses to ATP persisted in tetraethylammonium (1\u201010 mM) or tetrodotoxin (1\u20103 microM)\u2010containing solutions. 5. Quinine and quinidine (1\u2010100 microM) reversibly depressed both the ATP\u2010induced responses. Caffeine (100 microM), theophylline (100 microM) and 3\u2010isobutyl\u20101\u2010methylxanthine (1\u201010 microM) did not significantly affect the ATP\u2010induced depolarization but did reversibly depress the ATP\u2010induced hyperpolarization. 6. These results suggest that the ATP\u2010induced hyperpolarization may be due to activation, and the ATP\u2010induced depolarization to inactivation, of a calcium\u2010sensitive potassium conductance.<\/jats:p>","DOI":"10.1113\/jphysiol.1989.sp017464","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T04:10:34Z","timestamp":1418962234000},"page":"373-390","source":"Crossref","is-referenced-by-count":49,"title":["Adenosine 5'\u2010triphosphate modulates membrane potassium conductance in guinea\u2010pig myenteric neurones."],"prefix":"10.1113","volume":"408","author":[{"given":"Y","family":"Katayama","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Morita","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,1]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1989.sp017464","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1989.sp017464","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T04:53:53Z","timestamp":1697950433000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1989.sp017464"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,1]]}},"alternative-id":["10.1113\/jphysiol.1989.sp017464"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1989.sp017464","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,1]]}}}