{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T20:05:21Z","timestamp":1782331521762,"version":"3.54.5"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1989,2,1]],"date-time":"1989-02-01T00:00:00Z","timestamp":602294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1989,2]]},"abstract":"<jats:p>1. The effects of somatostatin and somatostatin analogues on a Ca2+ current from acutely isolated and short\u2010term (24\u201048 h) cultured adult rat superior cervical ganglion (SCG) neurones were studied using the whole\u2010cell variant of the patch\u2010clamp technique. 2. [D\u2010Trp8]Somatostatin (SOM) produced a rapid, reversible and concentration\u2010dependent reduction of the Ca2+ current. Ca2+ current amplitude was reduced over the voltage range \u201015 to +40 mV with the greatest reduction occurring where the amplitude was maximal (ca +10 mV). In the presence of SOM, the Ca2+ current rising phase was slower and biphasic at potentials between 0 and +40 mV. 3. Application of 0.1 microM\u2010SOM for greater than 10 s resulted in a desensitization of the response. During a 4 min application of 0.1 microM\u2010SOM, Ca2+ current amplitude returned to about 90% of control. A second application of 0.1 microM\u2010SOM produced less block than the initial application. 4. Concentration\u2010response curves for SOM, somatostatin\u201014 (SOM\u201014) and somatostatin\u201028 (SOM\u201028) were fitted to a single\u2010site binding isotherm. The concentrations producing half\u2010maximal block and the maximal attainable blocks of the Ca2+ current for SOM, SOM\u201014 and SOM\u201028 were 3.3, 5.4 and 35 nM, respectively and 55, 51 and 54%, respectively. SOM\u201014 and SOM\u201028 slowed the Ca2+ current rising phase in a manner similar to that of SOM. Somatostatin\u201028 had no effect on the Ca2+ current at 1 microM. 5. The magnitude of the Ca2+ current block produced by 0.1 microM\u2010SOM was not significantly altered in the presence of 1 microM\u2010idazoxan, atropine, naloxone or the somatostatin antagonist aminoheptanoyl\u2010Phe\u2010D\u2010Trp\u2010Lys\u2010O\u2010benzyl\u2010Thr. 6. Internal dialysis with solutions containing 500 microM\u2010guanylyl\u2010imidodiphosphate (Gpp(NH)p) or guanosine\u20105'\u2010O\u2010(3\u2010thiotriphosphate)(GTP\u2010gamma\u2010S) decreased the Ca2+ current amplitude by 36 and 41%, respectively, and induced a biphasic rising phase in the Ca2+ current. Under these conditions, application of 0.1 microM\u2010SOM produced significantly less block of Ca2+ current amplitude (7.1 and 14.7%, respectively) when compared with controls. 7. Internal dialysis with solutions containing 500 microM\u2010guanosine\u20105'\u2010O\u2010(2\u2010thiodiphosphate)(GDP\u2010beta\u2010S) had no significant effect on either the Ca2+ current amplitude or block produced by 0.1 microM\u2010SOM. 8. Internal dialysis with solutions containing 500 microM\u2010cyclic adenosine 3',5'\u2010monophosphate (cyclic AMP) and 3\u2010isobutyl\u20101\u2010methylxanthine had no significant effect on either the Ca2+ current block produced by 0.1 microM\u2010SOM or the Ca2+ current amplitude.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1989.sp017494","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T03:56:42Z","timestamp":1418961402000},"page":"221-240","source":"Crossref","is-referenced-by-count":140,"title":["Somatostatin blocks a calcium current in rat sympathetic ganglion neurones."],"prefix":"10.1113","volume":"409","author":[{"given":"S R","family":"Ikeda","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G G","family":"Schofield","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1989,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1989.sp017494","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1989.sp017494","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T14:03:36Z","timestamp":1697983416000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1989.sp017494"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,2]]}},"alternative-id":["10.1113\/jphysiol.1989.sp017494"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1989.sp017494","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,2]]}}}