{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T20:37:56Z","timestamp":1718656676880},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1989,5,1]],"date-time":"1989-05-01T00:00:00Z","timestamp":609984000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1989,5]]},"abstract":"<jats:p>1. When rabbit corneal endothelial cells are impaled with 3 M\u2010KCl\u2010filled microelectrodes (Rt = 20\u201070 M omega) a stable membrane potential of \u201028.7 +\/\u2010 4.8 mV (mean +\/\u2010 S.D., n = 400) is measured. 2. Varying the [KCl] of the filling solution causes a change in measured membrane potential; 154 mM gives typically \u20102 mV, 10 mM typically +37 mV. 3. Variation in membrane potential with different [KCl] cannot be ascribed to tip potential. Double\u2010barrelled microelectrodes containing a different [KCl] in each barrel both give the same membrane potential when inserted into a cell. 4. Microelectrodes filled with a reference liquid ion exchanger (RLIE) give a membrane potential of +2 mV. 5. Impaling a cell with a double\u2010barrelled microelectrode, one barrel containing KCl and the other RLIE, showed that the ion exchanger is not having a toxic effect on the cell and does not affect membrane potential measured by the KCl\u2010filled barrel. 6. We suggest that microelectrodes containing non\u2010isotonic concentrations of KCl generate a significant and artifactual change in membrane potential of corneal endothelial cells caused by the movement of excess KCl (originating from the microelectrode tip) across the plasma membrane where a liquid\u2010junction potential is generated. 7. We further suggest that the physiological membrane potential of corneal endothelial cells is around zero. This could result from a solution of the constant field equation where: 0.9 PNa congruent to PK congruent to 3.2 PCl.<\/jats:p>","DOI":"10.1113\/jphysiol.1989.sp017621","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T03:01:48Z","timestamp":1418958108000},"page":"365-374","source":"Crossref","is-referenced-by-count":5,"title":["A near\u2010zero membrane potential in transporting corneal endothelial cells of rabbit."],"prefix":"10.1113","volume":"412","author":[{"given":"S","family":"Hodson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Wigham","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1989.sp017621","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1989.sp017621","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T13:01:41Z","timestamp":1697979701000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1989.sp017621"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,5]]}},"alternative-id":["10.1113\/jphysiol.1989.sp017621"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1989.sp017621","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,5]]}}}