{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T09:44:45Z","timestamp":1698054285484},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1989,7,1]],"date-time":"1989-07-01T00:00:00Z","timestamp":615254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1989,7]]},"abstract":"<jats:p>1. The voltage\u2010activated outward currents in diencephalon\u2010derived neuroendocrine pineal cells, dissociated from rats aged 1 day to 3 weeks post\u2010natal, were studied with the whole\u2010cell variation of the patch\u2010clamp technique and compared with those of adult rats (1\u20103 months post\u2010natal). 2. Thirty\u2010five per cent of the 1\u2010week\u2010old cells displayed a single slowly inactivating outward current that had properties which distinguished it from the classical IA and IK currents. This current, named IK(d) for developmental, activated at potentials near \u201035 mV. Its time to half\u2010maximal activation (t 1\/2) ranged from 16 ms at \u201030 mV to 4 ms at + 15 mV. No other membrane currents were apparent with depolarizing steps up to +80 mV. 3. IK(d) displayed slow inactivation at depolarized potentials. The time constant for this inactivation was on the order of several hundred milliseconds. The curve for steady\u2010state inactivation disclosed that the current was 50% inactivated near \u201090 mV. This current was not found in cells dissociated from animals 4 or more weeks of age. 4. The reversal potential determined from the amplitude of the tail current at various repolarizing voltages was \u201076 mV. Tetraethylammonium and 4\u2010aminopyridine reduced the amplitude of the current. The amplitude and time course of this current was not affected by the removal of external Ca2+. Similarly, removal of Cl\u2010 did not affect the current characteristics. 5. Sixty\u2010five per cent of the 1\u2010week\u2010old cells displayed IA and IK. IK rose slowly with time and displayed a threshold of activation near \u201020 mV. No current decay was observed during a 160 ms pulse. IA activated with step potentials positive to \u201050 mV. This current rose faster than IK(d) and IK, and it had a significant decay over a 160 ms pulse. 6. IA and IK were observed as early as 1 day after birth. Comparison of the time course of activation of IA and IK from young and adult animals showed a small increase (2\u20103 ms at 0 mV) in the time to peak and half\u2010maximal current, respectively. With a step potential to \u201020 mV, the time constant of decay of IA increased from 34.6 ms in 2\u2010day\u2010old animals to 42.9 ms in adult animals. 7. The results indicate that unlike adult pineal cells, some cells from young animals express a kinetically distinct outward current (IK(d)) which was observed in the absence of IA and IK.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1989.sp017688","type":"journal-article","created":{"date-parts":[[2014,12,19]],"date-time":"2014-12-19T01:06:48Z","timestamp":1418951208000},"page":"283-300","source":"Crossref","is-referenced-by-count":15,"title":["Post\u2010natal development of K+ currents studied in isolated rat pineal cells."],"prefix":"10.1113","volume":"414","author":[{"given":"L G","family":"Aguayo","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1989.sp017688","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1989.sp017688","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T13:15:08Z","timestamp":1697980508000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1989.sp017688"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,7]]}},"alternative-id":["10.1113\/jphysiol.1989.sp017688"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1989.sp017688","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,7]]}}}