{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:18:44Z","timestamp":1698124724353},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1989,9,1]],"date-time":"1989-09-01T00:00:00Z","timestamp":620611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1989,9]]},"abstract":"<jats:p>1. Fragments of rat submandibular gland were pre\u2010loaded with 86Rb+, an isotopic marker of potassium transport, and rate constants for 86Rb+ efflux were determined during superfusion with a physiological salt solution. 2. In sodium\u2010containing solutions acetylcholine evoked a rapid and immediate increase in efflux rate. After reaching a peak value, the efflux rate initially declined rapidly, but a second, slowly declining phase to the response was also evident. The response could be resolved into Ca2(+)\u2010independent and Ca2(+)\u2010dependent phases. 3. The basal efflux rate was elevated during superfusion with solutions in which sodium had been replaced with either lithium or N\u2010methyl\u2010D\u2010glucammonium (NMDG+). Although lithium had a greater effect, which was absent under calcium\u2010free conditions, addition of calcium to initially calcium\u2010free, lithium\u2010containing solutions did not affect the rate of efflux. 4. In the presence of calcium the response to acetylcholine was augmented during exposure to lithium\u2010containing, sodium\u2010free solutions but, in contrast, slightly inhibited when NMDG+ was used as a sodium substituent. 5. The transient, calcium\u2010independent component of the response to acetylcholine was unaffected by exposure to lithium, whereas the calcium\u2010dependent phase of the response was inhibited. 6. Responsiveness to acetylcholine was reduced during superfusion with a calcium\u2010free, NMDG+\u2010containing solution. The response normally observed when extracellular Ca2+ was subsequently elevated, in the continued presence of acetylcholine, was also inhibited. Sensitivity to acetylcholine was retained, however, when the tissue was initially exposed to a solution containing approximately 20 mumol l\u20101 Ca2+. The response was smaller than that evoked in sodium\u2010containing solutions. 7. The use of lithium as a sodium substituent presents special problems, possibly related to the effects of this ion on the metabolic cycling of phosphatidylinositol\u20104,5\u2010bisphosphate metabolites.<\/jats:p>","DOI":"10.1113\/jphysiol.1989.sp017774","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T23:44:17Z","timestamp":1418946257000},"page":"503-515","source":"Crossref","is-referenced-by-count":6,"title":["Potassium (86Rb+) efflux from the rat submandibular gland under sodium\u2010free conditions in vitro."],"prefix":"10.1113","volume":"416","author":[{"given":"D L","family":"Bovell","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H Y","family":"Elder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J D","family":"Pediani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S M","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1989.sp017774","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1989.sp017774","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T00:47:47Z","timestamp":1697935667000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1989.sp017774"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,9]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,9]]}},"alternative-id":["10.1113\/jphysiol.1989.sp017774"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1989.sp017774","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,9]]}}}