{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T11:44:36Z","timestamp":1773661476370,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1989,12,1]],"date-time":"1989-12-01T00:00:00Z","timestamp":628473600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1989,12]]},"abstract":"<jats:p>1. The effect of the spider venom argiopine on L\u2010glutamate\u2010activated membrane channels of crayfish muscle was investigated using the patch\u2010clamp technique. 2. When 10(\u20102) M\u2010glutamate and 10(\u20109) M\u2010argiopine were contained in the pipette solution of a cell\u2010attached patch, bursts of openings of excitatory channels appeared after formation of the patch. These bursts ceased abruptly after variable periods of time in the range of 5 min. Higher concentrations of argiopine (up to 10(\u20106) M) blocked more rapidly, approximately in proportion to concentration. 3. The block of excitatory channels could be partially or completely reversed by hyperpolarizing the membrane by up to \u2010190 mV from the resting potential. The time constant of the recovery of channel opening decreased with increasing hyperpolarization and was 2 ms with \u2010160 mV hyperpolarization. Switching back from the hyperpolarized level to the resting potential, the time constant for the resulting block was about 3 s (10(\u20107) M\u2010argiopine). Potential\u2010dependent block by argiopine with similar characteristics was also observed in outside\u2010out patches. 4. Up to argiopine concentrations of 10(\u20107) M the kinetics of channel openings and of bursts measured in pre\u2010block periods or during reversal of the block by hyperpolarization were indistinguishable from controls. 5. When the potential\u2010dependent block observed in the presence of 10(\u20106) M\u2010argiopine and 10(\u20102) M\u2010glutamate was reversed by hyperpolarization, additional short closings occurred during bursts. This \u2018flickering block\u2019 did not change burst length appreciably, but an additional open time component (tau = 0.1 ms) appeared and the average open time per burst was reduced. 6. At least two reaction steps seem necessary to model the behaviour of the potential\u2010dependent block. The flickering block may be described as intermittent blocking of the channel which does not interfere with the reactions between glutamate and the channel.<\/jats:p>","DOI":"10.1113\/jphysiol.1989.sp017887","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T21:12:25Z","timestamp":1418937145000},"page":"569-587","source":"Crossref","is-referenced-by-count":25,"title":["Argiopine blocks glutamate\u2010activated single\u2010channel currents on crayfish muscle by two mechanisms."],"prefix":"10.1113","volume":"419","author":[{"given":"S M","family":"Antonov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J","family":"Dudel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Franke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Hatt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1989,12]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1989.sp017887","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1989.sp017887","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T17:23:54Z","timestamp":1697995434000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1989.sp017887"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1989,12]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1989,12]]}},"alternative-id":["10.1113\/jphysiol.1989.sp017887"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1989.sp017887","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1989,12]]}}}