{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,17]],"date-time":"2026-09-17T06:59:12Z","timestamp":1789628352803,"version":"build-2803163510"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,1,1]],"date-time":"1990-01-01T00:00:00Z","timestamp":631152000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1990,1]]},"abstract":"<jats:p>1. Bull\u2010frog sympathetic neurones in primary culture were voltage clamped in the whole\u2010cell configuration. The pipette solution contained ATP (5 mM). 2. A hyperpolarization\u2010activated sodium\u2010potassium current (H\u2010current: IH) was separated from other membrane currents in a nominally calcium\u2010free solution containing cobalt (2 mM), magnesium (4 mM), barium (2 mM), tetraethylammonium (20 mM), tetrodotoxin (3 microM), apamin (30 nM) and 4\u2010aminopyridine (1 mM). IH was selectively blocked by caesium (10\u2010300 microM). 3. The steady\u2010state activation of IH occurred between \u201060 and \u2010130 mV. The H\u2010conductance was 4.1\u20106.6 nS at the half\u2010activation voltage of \u201090 mV. With the concentrations of potassium and sodium ions in the superfusate at 20 and 70 mM, respectively, the reversal potential of IH was about \u201020 mV. IH was activated with a time constant of 2.8 s at \u201090 mV and 22 degrees C. The Q10 between 16 and 26 degrees C was 4.3. 4. A non\u2010hydrolysable ATP analogue in the pipette solution did not support IH activation. Intracellular \u2018loading\u2019 of GTP\u2010gamma\u2010S (30\u2010500 microM) led to a progressive activation of IH. 5. Forskolin (10 microM) increased the maximum conductance of IH by 70%. This was associated with a depolarizing shift in the half\u2010activation voltage (5\u201010 mV) and in the voltage dependence of the activation\/deactivation time constant of IH. 6. Essentially the same results as with forskolin were obtained by intracellular \u2018loading\u2019 with cyclic AMP (3\u201010 microM) or bath application of 8\u2010bromo cyclic AMP (0.1\u20101 mM), dibutyryl cyclic AMP (1 mM) and 3\u2010isobutyl\u20101\u2010methylxanthine (0.1\u20101 mM). 7. The protein kinase inhibitor H\u20108 (1\u201010 microM) decreased the peak amplitude of IH. Phorbol 12\u2010myristate 13\u2010acetate (10 microM), a protein kinase C activator, was without effect. 8. It is concluded that a voltage\u2010dependent cation current can be regulated by the basal activity of adenylate cyclase, presumably through protein kinase A, in vertebrate sympathetic neurones.<\/jats:p>","DOI":"10.1113\/jphysiol.1990.sp017920","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T15:28:53Z","timestamp":1418916533000},"page":"409-429","source":"Crossref","is-referenced-by-count":85,"title":["Cyclic AMP regulates an inward rectifying sodium\u2010potassium current in dissociated bull\u2010frog sympathetic neurones."],"prefix":"10.1113","volume":"420","author":[{"given":"T","family":"Tokimasa","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Akasu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1990,1]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1990.sp017920","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1990.sp017920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T06:59:47Z","timestamp":1697957987000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1990.sp017920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,1]]}},"alternative-id":["10.1113\/jphysiol.1990.sp017920"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1990.sp017920","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,1]]}}}