{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T19:29:39Z","timestamp":1790191779358,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,6,1]],"date-time":"1990-06-01T00:00:00Z","timestamp":644198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>1. The pharmacological sensitivities and physiological contributions of two types of Ca2(+)\u2010activated K+ channels (BK and SK) in GH3 cells were examined by the outside\u2010out, whole\u2010cell and cell\u2010attached modes of the patch\u2010clamp technique. 2. BK channels (250\u2010300 pS in symmetrical 150 mM\u2010K+) in outside\u2010out patches were blocked by external tetraethylammonium (TEA) and by 50 nM\u2010charybdotoxin (CTX), but were not blocked by apamin. 3. SK channels (9\u201014 pS in symmetrical 150 mM\u2010K+) in outside\u2010out patches were blocked by external TEA and by apamin, but were not blocked by 50 nM\u2010CTX. 4. The dissociation constant (Kd) for TEA block of SK channels (3.1 +\/\u2010 0.37 mM) was 12\u2010fold greater than the Kd for the BK channels (260 +\/\u2010 21 microM). The TEA blockade of both channels was not strongly voltage dependent: for both channels the TEA binding site sensed less than 20% of the membrane electric field. 5. Application of blockers of the BK channels (1 mM\u2010TEA and 50 nM\u2010CTX) to whole cells under current clamp prolonged action potential duration; whereas application of apamin, a selective blocker of the SK channel, inhibited a slowly decaying after\u2010hyperpolarization and had little effect on action potential duration. Apamin also increased the firing rate in 30% of the spontaneously pacing cells. 6. It is suggested that BK channels contribute to action potential repolarization: whereas SK channels contribute to the regulation of action potential firing rate.<\/jats:p>","DOI":"10.1113\/jphysiol.1990.sp018095","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T11:46:57Z","timestamp":1418903217000},"page":"117-132","source":"Crossref","is-referenced-by-count":98,"title":["Tetraethylammonium blockade of apamin\u2010sensitive and insensitive Ca2(+)\u2010activated K+ channels in a pituitary cell line."],"prefix":"10.1113","volume":"425","author":[{"given":"D G","family":"Lang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A K","family":"Ritchie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1990,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1990.sp018095","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1990.sp018095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T07:24:08Z","timestamp":1698045848000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1990.sp018095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1113\/jphysiol.1990.sp018095"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1990.sp018095","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]}}}