{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T09:49:31Z","timestamp":1698140971398},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1990,6,1]],"date-time":"1990-06-01T00:00:00Z","timestamp":644198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1990,6]]},"abstract":"<jats:p>1. Phorbol esters are known to inhibit phospholipase C\u2010mediated hydrolysis of membrane phosphoinositide. This inhibition is attributed to participation of protein kinase C (PKC) in a negative\u2010feedback control of phosphoinositide metabolism. We have tested this hypothesis by using different types of activators and inhibitors of PKC. 2. Phorbol\u201012,13\u2010dibutyrate (PDB) inhibited the stimulatory effect of acetylcholine (ACh) on [3H]inositol monophosphate ([3H]IP) formation in cultured sympathetic neurons of the chick embryo and adrenal medulla of the rat. 3. Acetylcholine (ACh) and 5\u2010hydroxytryptamine (5\u2010HT) activated neuronal PKC by 3\u2010 to 8\u2010fold. The extent of PKC activation by 100 microM\u2010ACh was comparable to that of 100 nM\u2010PDB. Activation of PKC by pre\u2010incubation of sympathetic neurons with ACh (or 5\u2010HT) did not inhibit the stimulatory effects of ACh (or 5\u2010HT) on [3H]IP formation. 4. Pre\u2010treatment of sympathetic neurons or adrenal medulla with a PKC inhibitor H7 (1\u2010(5\u2010isoquinolinyl\u2010sulphonyl)\u20102\u2010methyl\u2010piperazine) almost completely blocked activation of the enzyme induced by PDB, ACh or 5\u2010HT. However, blockade of PKC did not prevent the inhibitory effects of PDB on ACh\u2010induced [3H]IP formation. 5. Vasoactive intestinal polypeptide (VIP) and muscarine induced catecholamine secretion from the perfused adrenal medulla via formation of inositol\u20101,4,5\u2010tirisphosphate (IP3). Phorbol\u201012,13\u2010dibutyrate decreased muscarine\u2010induced catecholamine secretion. However, activation of PKC by VIP had no effect on muscarine\u2010induced catecholamine secretion and vice versa. 6. These results suggest that PKC is not negatively coupled to phosphoinositide hydrolysis in sympathetic neurons and chromaffin cells. Phorbol esters must have targets other than PKC to interfere with the phosphoinositide hydrolysis.<\/jats:p>","DOI":"10.1113\/jphysiol.1990.sp018106","type":"journal-article","created":{"date-parts":[[2014,12,18]],"date-time":"2014-12-18T11:46:57Z","timestamp":1418903217000},"page":"335-346","source":"Crossref","is-referenced-by-count":2,"title":["Phosphoinositide hydrolysis is not negatively regulated by protein kinase C in the peripheral tissues of rat and chick."],"prefix":"10.1113","volume":"425","author":[{"given":"S V","family":"Bhave","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R K","family":"Malhotra","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T D","family":"Wakade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Wakade","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1990,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1990.sp018106","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1990.sp018106","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T07:23:53Z","timestamp":1698045833000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1990.sp018106"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1990,6]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1990,6]]}},"alternative-id":["10.1113\/jphysiol.1990.sp018106"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1990.sp018106","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1990,6]]}}}