{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,20]],"date-time":"2026-02-20T23:22:59Z","timestamp":1771629779655,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1991,3,1]],"date-time":"1991-03-01T00:00:00Z","timestamp":667785600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1991,3]]},"abstract":"<jats:p>1. The whole\u2010cell configuration of the patch\u2010clamp technique has been used to investigate the conductance properties of villus enterocytes isolated from guinea\u2010pig small intestinal epithelium. 2. With near physiological ionic gradients inward and outward rectification was observed in the hyperpolarizing and depolarizing voltage domains respectively. 3. Replacement of intra\u2010 and extracellular K+ with N\u2010methyl\u2010D\u2010glucamine (NMG) eliminated inward rectification but did not alter outward currents. In symmetrical low Cl\u2010 solutions outward currents were reduced but inward rectification was not affected. Under these conditions increases in extracellular K+ shifted both the current\u2010voltage relation and the extrapolated reversal potential as expected for a K(+)\u2010selective current. 4. The inwardly rectifying nature of the K+ current observed here remained unaltered after chelation of internal Mg2+ with ATP or EDTA. 5. Extracellular application of 5 mM\u2010Ba2+ or 50 micrograms ml\u20101 of the venom of the scorpion Leiurus quinquestriatus abolished the inward K+ current, while 5 mM\u2010extracellular tetraethylammonium (TEA) had little effect. 6. The current remaining in the presence of symmetrical Cl\u2010 solutions and in the complete absence of K+ rectified outwardly and reversed at 0 mV. The anionic nature of this current was confirmed by replacing Cl\u2010 with different anions. SCN\u2010 and Br\u2010 carried more current than Cl\u2010, while F\u2010 and gluconate were less permeant. 7. Anionic currents of villus guinea\u2010pig enterocytes were not stimulated by cyclic AMP and were strongly and reversibly inhibited by the Cl\u2010 channel blocker 5\u2010nitro\u20102\u2010(3\u2010phenylpropylamino) benzoic acid (NPPB, 10(\u20105) M). 8. The inwardly rectifying K+ current described here shares some, but not all, characteristics with others previously described. It is postulated that this conductance might function to couple K+ permeability and the Na(+)\u2010K+ pump rate in enterocytes. Absorption of chloride may be mediated by the Cl\u2010 channels.<\/jats:p>","DOI":"10.1113\/jphysiol.1991.sp018474","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T23:38:19Z","timestamp":1418859499000},"page":"351-367","source":"Crossref","is-referenced-by-count":19,"title":["K+ and Cl\u2010 currents in enterocytes isolated from guinea\u2010pig small intestinal villi."],"prefix":"10.1113","volume":"434","author":[{"given":"F V","family":"Sep\u00falveda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F","family":"Fargon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P A","family":"McNaughton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,3]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1991.sp018474","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1991.sp018474","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,22]],"date-time":"2023-10-22T23:37:38Z","timestamp":1698017858000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1991.sp018474"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,3]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,3]]}},"alternative-id":["10.1113\/jphysiol.1991.sp018474"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018474","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,3]]}}}