{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,4]],"date-time":"2025-11-04T22:27:57Z","timestamp":1762295277024},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1991,4,1]],"date-time":"1991-04-01T00:00:00Z","timestamp":670464000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1991,4]]},"abstract":"<jats:p>1. Cultured bull\u2010frog dorsal root ganglion cells were voltage clamped in the whole\u2010cell configuration. The cells were superfused with a nominally calcium\u2010free Ringer solution containing tetrodotoxin (3 microM), magnesium (10 mM), cobalt (1 mM), barium (2 mM), 4\u2010aminopyridine (3 mM) and caesium (2 mM). 2. Step depolarizations (10\u201040 mV, 100\u2010300 ms) from a holding potential close to the rest (typically \u201070 mV) evoked an outward current (IK) followed by an outward tail current. The peak amplitude of the current was reduced to less than 10% by tetraethylammonium (30 mM). 3. IK developed to its peak in 200 ms at \u201030 mV. Tail currents reversed at potentials that changed according to the logarithm of the extracellular potassium concentrations. 4. Tail currents declined to the baseline according to an exponential function of time (tau congruent to 40 ms at \u201060 mV) and its reciprocal time constant increased e\u2010fold with a 13 mV hyperpolarization. 5. The current inactivated during sustained (1\u201020 s) depolarizing pulses according to a single exponential function (tau congruent to 3 s). 6. The peak amplitude of IK at \u201030 mV was progressively increased as the holding potential was made more negative than \u201070 mV reaching the maximum with step depolarizations from \u2010120 mV. Reversed phenomenon was observed as the holding potential was made less negative than \u201070 mV. 7. The removal of the steady\u2010state inactivation occurred along with a single exponential function and the time constant was decreased from 70 ms at \u201070 mV to 10 ms at \u2010120 mV. 8. It is suggested that a slowly inactivating potassium current which we called IK in amphibian sensory neurones could be a class of a \u2018delayed\u2019 rectifier potassium current. A potassium current with properties indistinguishable from those which have been described for the sensory IK also occurred in cultured bull\u2010frog sympathetic neurones. 9. Forskolin (1\u201030 microM) and 1,9\u2010dideoxy forskolin (10 microM) reduced the amplitude of IK by up to 85% but these actions were not mimicked by any of 8\u2010bromo\u2010cyclic AMP (1 mM), dibutyryl cyclic AMP (1 mM) and 3\u2010isobutyl\u20101\u2010methylxanthine (1 mM). A hydrophilic forskolin analogue, 7\u2010O\u2010hemisuccinyl\u20107\u2010deacetyl forskolin (10 microM), was about one\u2010tenth as potent as forskolin (10 microM).<\/jats:p>","DOI":"10.1113\/jphysiol.1991.sp018527","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T23:18:54Z","timestamp":1418858334000},"page":"585-604","source":"Crossref","is-referenced-by-count":25,"title":["Slowly inactivating potassium current in cultured bull\u2010frog primary afferent and sympathetic neurones."],"prefix":"10.1113","volume":"435","author":[{"given":"T","family":"Tokimasa","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Tsurusaki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Akasu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1991.sp018527","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1991.sp018527","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T04:58:41Z","timestamp":1698037121000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1991.sp018527"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,4]]}},"alternative-id":["10.1113\/jphysiol.1991.sp018527"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018527","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,4]]}}}