{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T23:53:26Z","timestamp":1792713206573,"version":"4.5.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1991,5,1]],"date-time":"1991-05-01T00:00:00Z","timestamp":673056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1991,5]]},"abstract":"<jats:p>1. The dual\u2010emission pH\u2010sensitive fluoroprobe carboxy\u2010SNARF\u20101 (carboxy\u2010seminaptharhodofluor) was used to measure pHi in type I cells enzymically dispersed from the neonatal rat carotid body. 2. Steady\u2010state pHi in cells bathed in a HEPES\u2010buffered Tyrode solution (pH 7.4) was found to be remarkably alkaline (pHi = 7.77) whereas cells bathed in a CO2\u2010HCO3(\u2010)\u2010buffered Tyrode solution (pH 7.4) had a more \u2018normal\u2019 pHi (pHi = 7.28). These observations were further substantiated by using an independent nullpoint test method to determine pHi. 3. Intracellular intrinsic buffering (beta, determined by acidifying the cell using an NH4Cl pre\u2010pulse) was in the range 7\u201020 mM per pH unit and appeared to be dependent upon pHi with beta increasing as pHi decreased. 4. In cells bathed in a HEPES\u2010buffered Tyrode solution, pHi recovery from an induced intracellular acid load (10 mM\u2010NH4Cl pre\u2010pulse) was inhibited by the Na(+)\u2010H+ exchange inhibitor ethyl isopropyl amiloride (EIPA; 150 microM) or substitution of Nao+ with N\u2010methyl\u2010D\u2010glucamine (NMG). Both EIPA and Nao+ removal also caused a rapid intracellular acidification, which in the case of Nao+ removal, was readily reversible. The rate of this acidification was similar for both Nao+ removal and EIPA addition. 5. Transferring cells from a HEPES\u2010buffered Tyrode solution to one buffered with 5% CO2\u2010HCO3\u2010 resulted in an intracellular acidification which was partially, or wholly, sustained. The rate of acidification upon transfer to CO2\u2010HCO3\u2010 was considerably slowed by the membrane permeant carbonic anhydrase inhibitor, acetazolamide, thus indicating the presence of the enzyme in these cells. 6. In CO2\u2010HCO3(\u2010)\u2010buffered Tyrode solution, pHi recovery from an intracellular acidosis (NH4+ pre\u2010pulse) was only partially inhibited by EIPA or amiloride whereas Nao+ removal completely inhibited the recovery. The stilbene DIDS (4,4\u2010diisothiocyanatostilbenedisulphonic acid, 200 microM) also partially inhibited pHi recovery following an induced intracellular acidosis. Furthermore, the pre\u2010treatment with 200 microM\u2010DIDS of a pre\u2010acidified cell in Na(+)\u2010free Tyrode solution completely inhibited pHi recovery when Nao+ was reintroduced together with concomitant addition of 150 microM\u2010EIPA. We conclude, that in the presence of CO2\u2010HCO3\u2010, a Na(+)\u2010 and HCO3\u2010dependent (DIDS inhibitable) mechanism aids acid extrusion.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1991.sp018542","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T23:12:34Z","timestamp":1418857954000},"page":"107-129","source":"Crossref","is-referenced-by-count":99,"title":["Intracellular pH and its regulation in isolated type I carotid body cells of the neonatal rat."],"prefix":"10.1113","volume":"436","author":[{"given":"K J","family":"Buckler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R D","family":"Vaughan\u2010Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C","family":"Peers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P C","family":"Nye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1991,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1991.sp018542","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1991.sp018542","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T05:27:52Z","timestamp":1698038872000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1991.sp018542"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,5]]}},"alternative-id":["10.1113\/jphysiol.1991.sp018542"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018542","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,5]]}}}