{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T02:56:16Z","timestamp":1773802576191,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1991,7,1]],"date-time":"1991-07-01T00:00:00Z","timestamp":678326400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1991,7]]},"abstract":"<jats:p>1. The membrane currents evoked by glutamate agonists on isolated and identified neurones of molluscan pedal ganglia were investigated using the voltage clamp technique. 2. The fast chloride current (Er (reversal potential) = \u201041 mV) evoked in a Ped\u20109 neurone by application of glutamate, quisqualate and ibotenic acid could be blocked by furosemide (0.1 mM). The slow potassium current (Er = \u201085 mV) evoked in Ped\u20108 and Ped\u20109 neurones by glutamate, quisqualate and kainate could be blocked by tetraethylammonium (50 microM). 3. N\u2010Methyl\u2010D\u2010aspartate (NMDA) and alpha\u2010amino\u20103\u2010hydroxy\u20105\u2010methyl\u20104\u2010isoxazole\u2010proprionic acid (AMPA) failed to induce a response in neurones studies. 4. The spider venoms argiopine and argiopinine III (50\u2010500 nM) selectively inhibited quisqualate\u2010induced potassium current, but had no influence on glutamate\u2010, ibotenate\u2010 or quisqualate\u2010induced chloride and kainate\u2010induced potassium currents. Glutamate\u2010induced potassium current was partially inhibited by argiopine and argiopinine III. 5. The existence of several types of distinct glutamate receptors was confirmed in cross\u2010desensitization experiments, and a lack of interaction was observed between quisqualate and kainate. 6. Potassium currents induced both by quisqualate and kainate strongly depended on temperature and could be blocked by pertussis toxin. Intracellular injection of the calcium chelator, EGTA, did not affect quisqualate and kainate responses. 7. In neurones loaded with non\u2010hydrolysable GTP analogues, GTP\u2010gamma\u2010S (guanosine\u20105'\u2010O\u2010(3\u2010thio)triphosphate) or GppNHp (5'\u2010guanylylimidodiphosphate), the potassium current was gradually induced in the absence of agonists. As this current progressed, the magnitude of the glutamate\u2010 or kainate\u2010evoked current transients became smaller and finally negligible. The GTP\u2010gamma\u2010S\u2010induced current was not inhibited by argiopine. 8. These data indicate that in the molluscan neurones studied there are at least three pharmacologically distinct glutamate receptors: (1) a receptor of quisqualate\u2010ibotenate type which directly controls chloride channel; (2) quisqualate and (3) kainate receptors which control in calcium\u2010independent manner the common potassium channel by activation of GTP\u2010binding protein.<\/jats:p>","DOI":"10.1113\/jphysiol.1991.sp018654","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T19:47:21Z","timestamp":1418845641000},"page":"15-35","source":"Crossref","is-referenced-by-count":46,"title":["Different types of glutamate receptors in isolated and identified neurones of the mollusc Planorbarius corneus."],"prefix":"10.1113","volume":"439","author":[{"given":"V Yu","family":"Bolshakov","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S A","family":"Gapon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L G","family":"Magazanik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,7]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1991.sp018654","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1991.sp018654","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T15:46:52Z","timestamp":1698076012000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1991.sp018654"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,7]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,7]]}},"alternative-id":["10.1113\/jphysiol.1991.sp018654"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018654","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,7]]}}}