{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T20:28:38Z","timestamp":1718656118253},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1991,8,1]],"date-time":"1991-08-01T00:00:00Z","timestamp":681004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1991,8]]},"abstract":"<jats:p>1. Single guinea\u2010pig ventricular cells were voltage clamped using the patch clamp method combined with the pipette\u2010perfusion technique. The voltage\u2010dependent current systems were mostly blocked, and the background membrane conductance was measured by applying ramp pulses. 2. beta\u2010Adrenergic effectors and related substances such as adrenaline, isoprenaline, forskolin or internal application of cyclic AMP induced a current component which showed a reversal potential near the expected Cl\u2010 equilibrium potential as well as an outward rectification in the I\u2010V relation. It is suggested that the activation of this Cl\u2010 current was due to phosphorylation of the channel protein or related structure by the cyclic AMP\u2010dependent protein kinase. Coincidentally with the activation of the Cl\u2010 current, the membrane capacitance of the cell decreased reversibly. 3. Acetylcholine (ACh) depressed the responses induced by beta\u2010adrenergic stimulation and forskolin, but failed to interfere with the one induced by cyclic AMP. 4. The dose dependence of the Cl\u2010 current activation by isoprenaline or forskolin was fitted by the Hill equation, with a coefficient of 1.9 and a half\u2010maximum concentration K 1\/2 = 13 nM for isoprenaline, and with a Hill coefficient of 3 and a K 1\/2 = 1.2 microM for forskolin. In the presence of 5.5 microM\u2010ACh the dose\u2010response relation shifted to higher doses; K 1\/2 was 65 nM for isoprenaline and 3.6 microM for forskolin. 5. Washing out ACh in the presence of isoprenaline frequently caused transient overshoots of the response. When a saturating concentration of isoprenaline was used, this rebound was not observed. 6. The internal application of cyclic GMP enhanced the response of the Cl\u2010 current induced by isoprenaline or adrenaline. 7. When cyclic AMP was applied internally, the response was small in most cells. When the cell was superfused with 20 microM\u2010IBMX (3\u2010isobutyl\u20101\u2010methylxanthine), the Cl\u2010 current was consistently induced by the application of cyclic AMP. It is suggested that phosphodiesterase activity strongly buffered the influx of cyclic AMP through the patch pipette tip. 8. We suggest that the compensatory interaction between the beta\u2010adrenergic stimulation and the muscarinic inhibition is at the membrane level, most probably via GTP\u2010binding proteins in activating adenylate cyclase.<\/jats:p>","DOI":"10.1113\/jphysiol.1991.sp018705","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T17:57:08Z","timestamp":1418839028000},"page":"225-241","source":"Crossref","is-referenced-by-count":38,"title":["Beta\u2010adrenergic and muscarinic regulation of the chloride current in guinea\u2010pig ventricular cells."],"prefix":"10.1113","volume":"440","author":[{"given":"F M","family":"Tareen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Ono","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Noma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Ehara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1991,8]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1991.sp018705","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1991.sp018705","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T00:39:26Z","timestamp":1698021566000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1991.sp018705"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1991,8]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1991,8]]}},"alternative-id":["10.1113\/jphysiol.1991.sp018705"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1991.sp018705","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1991,8]]}}}