{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T05:20:31Z","timestamp":1698124831066},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,4,1]],"date-time":"1992-04-01T00:00:00Z","timestamp":702086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:p>1. This study was undertaken with an isolated vascularly perfused rat duodenojejunal preparation to investigate the mechanisms of the release of cholecystokinin measured by immunoassay (cholecystokinin\u2010like immunoreactivity, CCK\u2010LI). 2. Intra\u2010arterial infusion of forskolin (2\u201020 microM) evoked a prompt and well\u2010sustained secretion of CCK\u2010LI which was increased to a mean value of 600% of basal with the highest dose tested. 3\u2010Isobutyl\u20101\u2010methylxanthine (IBMX) (10(\u20106)\u201010(\u20104) M) stimulated the secretion of CCK\u2010LI (maximal increase of 400% of basal at 10(\u20104) M). 3. Intra\u2010arterial infusion of beta\u2010phorbol 12\u2010myristate 13\u2010acetate (5 x 10(\u20107)\u20105 x 10(\u20106) M) and calcium ionophore A23187 (10(\u20106)\u201010(\u20105) M) alone or in combination provoked only a transient increase in the release of CCK\u2010LI. 4. Luminal infusion of a 5% ovalbumin hydrolysate solution produced an immediate release of CCK\u2010LI followed by a well\u2010sustained secretion at 580% of basal. Intra\u2010arterial infusion of IBMX (10(\u20105) or 10(\u20104) M) in combination with luminal peptone induced a release of CCK\u2010LI which was equal to the sum of the CCK responses evoked by IBMX and peptone given separately. 5. Intra\u2010arterial infusion of EGTA (2 mM) abolished the forskolin\u2010 and peptone\u2010induced CCK secretion while luminal EGTA (2 mM) had no inhibitory effect. Verapamil (5 x 10(\u20105)\u201010(\u20104) M) or nifedipine (10(\u20105)\u20105 x 10(\u20105) M) inhibited the peptone\u2010evoked CCK secretion. A high concentration of trifluoperazine (10(\u20104) M) strongly reduced the release of CCK\u2010LI induced by intraluminal peptone while 10(\u20105) M was ineffective. 6. It is concluded that the peptone\u2010induced secretion of CCK\u2010LI involves a cyclic AMP\u2010dependent mechanism and the activation of calcium channels possibly located at the basolateral side of the CCK cell.<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019073","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T16:24:46Z","timestamp":1418833486000},"page":"37-48","source":"Crossref","is-referenced-by-count":5,"title":["Role of cyclic nucleotides and calcium in the nutrient\u2010induced release of cholecystokinin\u2010like immunoreactivity in rats."],"prefix":"10.1113","volume":"449","author":[{"given":"J C","family":"Cuber","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Aucouturier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C","family":"Bernard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Chayvialle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019073","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019073","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T17:14:14Z","timestamp":1698081254000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019073"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019073"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019073","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]}}}