{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:52:21Z","timestamp":1790895141331,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,4,1]],"date-time":"1992-04-01T00:00:00Z","timestamp":702086400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,4]]},"abstract":"<jats:p>1. Resting and acetylcholine\u2010induced membrane currents were measured in single mouse submandibular acinar cells using the patch\u2010clamp whole\u2010cell current recording technique. 2. Micromolar ACh activated a large, sustained outward, Ca(2+)\u2010dependent K+ current and a single transient inward Ca(2+)\u2010dependent C1\u2010current. 3. Nanomolar ACh induced a series of transients in both the K+ and C1\u2010 currents; C1\u2010 current activation was now observed throughout the period of agonist application. We consider this repetitive transient current activation better able to support sustained fluid and electrolyte secretion than the response elicited by a high dose of agonist. 4. Repetitive K+ and C1\u2010 current transients were also induced by 1 mM\u2010caffeine, consistent with caffeine\u2010induced Ca2+ release from the Ca(2+)\u2010sensitive Ca2+ stores which are thought to comprise part of the pathway for activation of secretion. 5. The ACh\u2010induced current transients were inhibited by 10 mM\u2010caffeine, 100 microM\u2010IBMX and 10 microM membrane\u2010permeable cyclic AMP. Therefore, it seems likely that caffeine is able to inhibit agonist\u2010induced calcium mobilization via a cyclic AMP\u2010dependent pathway.<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019077","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T16:24:46Z","timestamp":1418833486000},"page":"109-120","source":"Crossref","is-referenced-by-count":38,"title":["Acetylcholine\u2010 and caffeine\u2010evoked repetitive transient Ca(2+)\u2010activated K+ and C1\u2010 currents in mouse submandibular cells."],"prefix":"10.1113","volume":"449","author":[{"given":"P M","family":"Smith","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D V","family":"Gallacher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1992,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019077","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019077","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T17:14:10Z","timestamp":1698081250000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019077"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,4]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019077"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019077","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,4]]}}}