{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T22:30:57Z","timestamp":1773268257448,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,6,1]],"date-time":"1992-06-01T00:00:00Z","timestamp":707356800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,6]]},"abstract":"<jats:p>1. Mechanical recordings were made in vitro from circularly oriented strips of the bladder neck muscle of sheep. In the absence of drugs, electrical field stimulation at frequencies of 0.2\u20101 Hz evoked clear\u2010cut relaxations throughout 1 min stimulation periods, while higher stimulus frequencies (2\u20108 Hz) evoked variable responses consisting of relaxation, contraction or a mixture of both. All of the responses were abolished by tetrodotoxin (10(\u20106) M). 2. The contractions were reduced by guanethidine (10(\u20106) M) and atropine (10(\u20106) M), so that in the presence of these drugs clear\u2010cut relaxations were obtained at 0.2\u20108 Hz stimulation, indicating that the relaxations were mediated by non\u2010adrenergic, non\u2010cholinergic (NANC) nerves. 3. The NANC relaxations were blocked by L\u2010NG nitro arginine methyl ester (L\u2010NAME), an inhibitor of nitric oxide synthesis. The antagonism by L\u2010NAME was reversed by L\u2010arginine. 4. Another feature of the NANC relaxation was \u2018rebound contraction\u2019 which occurred when the stimulus was switched off. The rebound contraction was also blocked by L\u2010NAME and restored by L\u2010arginine. 5. The relaxations and rebound contractions were unaffected by either alpha,beta\u2010methylene ATP (10(\u20105) M) or 2\u2010methylthio ATP (10(\u20105) M). 6. S\u2010Nitroso\u2010L\u2010cysteine, a substance which spontaneously releases NO at physiological pH, mimicked the relaxation and rebound contraction produced by nerve stimulation. 7. It is concluded that nerve\u2010evoked relaxation of the bladder neck is mediated by NO, or a closely related substance such as S\u2010nitroso\u2010L\u2010cysteine.<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019157","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T16:12:53Z","timestamp":1418832773000},"page":"133-144","source":"Crossref","is-referenced-by-count":81,"title":["Mediation by nitric oxide of neurogenic relaxation of the urinary bladder neck muscle in sheep."],"prefix":"10.1113","volume":"451","author":[{"given":"K D","family":"Thornbury","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M A","family":"Hollywood","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N G","family":"McHale","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019157","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019157","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T12:28:46Z","timestamp":1698064126000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019157"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,6]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,6]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019157"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019157","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,6]]}}}