{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T05:40:40Z","timestamp":1790487640601,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,10,1]],"date-time":"1992-10-01T00:00:00Z","timestamp":717897600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,10]]},"abstract":"<jats:p>1. Whole\u2010cell K+ currents contributing to the resting membrane potential and repolarization of the action potential were studied in voltage\u2010clamped parasympathetic neurones dissociated from neonatal rat intracardiac ganglia and maintained in tissue culture. 2. Rat intracardiac neurones had a mean resting membrane potential of \u201052 mV and mean input resistance of 850 M omega. The current\u2010voltage relationship recorded during slow voltage ramps indicated the presence of both leakage and voltage\u2010dependent currents. The contribution of Na+, K+ and Cl\u2010 to the resting membrane potential was examined and relative ionic permeabilities PNa\/PK = 0.12 and PCl\/PK &lt; 0.001 were calculated using the Goldman\u2010Hodgkin\u2010Katz voltage equation. Bath application of the potassium channel blockers, tetraethylammonium ions (TEA; 1 mM) or Ba2+ (1 mM) depolarized the neurone by approximately 10 mV. Inhibition of the Na(+)\u2010K+ pump by exposure to K(+)\u2010free medium or by the addition of 0.1 mM ouabain to the bath solution depolarized the neurone by 3\u20105 mV. 3. In most neurones, depolarizing current pulses (0.5\u20101 s duration) elicited a single action potential of 85\u2010100 mV, followed by an after\u2010hyperpolarization of 200\u2010500 ms. In 10\u201015% of the neurones, sustained current injection produced repetitive firing at maximal frequency of 5\u20108 Hz. 4. Tetrodotoxin (TTX; 300 nM) reduced, but failed to abolish, the action potential. The magnitude and duration of the TTX\u2010insensitive action potential increased with the extracellular Ca2+ concentration, and was inhibited by bath application of 0.1 mM Cd2+. The repolarization rate of the TTX\u2010insensitive action potential was reduced, and after\u2010hyperpolarization was replaced by after\u2010depolarization upon substitution of internal K+ by Cs+. The after\u2010hyperpolarization of the action potential was reduced by bath application of Cd2+ (0.1 mM) and abolished by the addition of Cd2+ and TEA (10 mM). 5. Depolarization\u2010activated outward K+ currents were isolated by adding 300 nM TTX and 0.1 mM Cd2+ to the external solution. The outward currents evoked by step depolarizations increased to a steady\u2010state plateau which was maintained for &gt; 5 s. The instantaneous current\u2010voltage relationship, examined under varying external K+ concentrations, was linear, and the reversal (zero current) potential shifted in accordance with that predicted by the Nernst equation for a K(+)\u2010selective electrode. The shift in reversal potential of the tail currents as a function of the extracellular K+ concentration gave a relative permeability, PNa\/PK = 0.02 for the delayed outward K+ channel(s).(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019343","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T15:27:34Z","timestamp":1418830054000},"page":"405-424","source":"Crossref","is-referenced-by-count":59,"title":["Resting membrane potential and potassium currents in cultured parasympathetic neurones from rat intracardiac ganglia."],"prefix":"10.1113","volume":"456","author":[{"given":"Z J","family":"Xu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D J","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1992,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019343","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019343","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T13:21:09Z","timestamp":1698153669000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019343"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,10]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,10]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019343"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019343","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,10]]}}}