{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T03:31:35Z","timestamp":1771644695012,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,11,1]],"date-time":"1992-11-01T00:00:00Z","timestamp":720576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:p>1. Nerve terminals of the rat posterior pituitary were acutely dissociated and identified using a combination of morphological and immunohistochemical techniques. Terminal membrane currents were studied using the \u2018whole\u2010cell\u2019 patch clamp technique and channels were studied using inside\u2010out and outside\u2010out patches. 2. In physiological solutions, but with 7 mM 4\u2010aminopyridine (4\u2010AP), depolarizing voltage clamp steps from different holding potentials (\u201090 or \u201050 mV) elicited a fast, inward current followed by a slow, sustained, outward current. This outward current did not appear to show any steady\u2010state inactivation. 3. The threshold for activation of the outward current was \u201030 mV and the current\u2010voltage relation was \u2018bell\u2010shaped\u2019. The amplitude increased with increasingly depolarized potential steps. The outward current reversal potential was measured using tail current analysis and was consistent with that of a potassium current. 4. The sustained potassium current was determined to be dependent on the concentration of intracellular calcium. Extracellular Cd2+ (80 microM), a calcium channel blocker, also reversibly abolished the outward current. 5. The current was delayed in onset and was sustained over the length of a 150 ms\u2010duration depolarizing pulse. The outward current reached a peak plateau and then decayed slowly. The decay was fitted by a single exponential with a time constant of 9.0 +\/\u2010 2.2 s. The decay constants did not show a dependence on voltage but rather on intracellular Ca2+. The time course of recovery from this decay was complex with full recovery taking &gt; 190 s. 6. 4\u2010AP (7 mM), dendrotoxin (100 nM), apamin (40\u201080 nM), and charybdotoxin (10\u2010100 nM) had no effect on the sustained outward current. In contrast Ba2+ (200 microM) and tetraethylammonium inhibited the current, the latter in a dose\u2010dependent manner (apparent concentration giving 50% of maximal inhibition (IC50) = 0.51 mM). 7. The neurohypophysial terminal outward current recorded here corresponds most closely to a Ca(2+)\u2010activated K+ current (IK(Ca)) and not to a delayed rectifier or IA\u2010like current. It also has properties different from that of the Ca(2+)\u2010dependent outward current described in the magnocellular neuronal cell bodies of the hypothalamus. 8. A large conductance channel is often observed in isolated rat neurohypophysial nerve terminals. The channel had a unit conductance of 231 pS in symmetrical 150 mM K+.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019364","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T15:20:54Z","timestamp":1418829654000},"page":"47-74","source":"Crossref","is-referenced-by-count":47,"title":["A novel large\u2010conductance Ca(2+)\u2010activated potassium channel and current in nerve terminals of the rat neurohypophysis."],"prefix":"10.1113","volume":"457","author":[{"given":"G","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Thorn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J R","family":"Lemos","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019364","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019364","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:00:06Z","timestamp":1698055206000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019364"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,11]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019364"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019364","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11]]}}}