{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T11:08:13Z","timestamp":1789902493624,"version":"4.0.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,11,1]],"date-time":"1992-11-01T00:00:00Z","timestamp":720576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:p>1. Voltage\u2010clamp recordings using a single microelectrode were obtained from pyramidal neurones of the basolateral amygdala (BLA) in slices of the rat ventral forebrain. Slow inward current relaxations during hyperpolarizing voltage steps from a holding potential of \u201040 mV were identified as the muscarinic\u2010sensitive M\u2010current (IM), a time\u2010 and voltage\u2010dependent potassium current previously identified in other neuronal cell types. 2. Activation of IM was voltage dependent with a threshold of approximately \u201070 mV. At membrane potentials positive to this, the steady\u2010state current\u2010voltage (I\u2010V) relationship showed substantial outward rectification, reflecting the time\u2010 and voltage\u2010dependent opening of M\u2010channels. The underlying conductance (gM) also increased sharply with depolarization. 3. The reversal potential for IM was \u201084 mV in medium containing 3.5 mM K+. This was shifted positively by 27 mV when the external K+ concentration was raised to 15 mM. 4. The time courses of M\u2010current activation and deactivation were fitted by a single exponential. The time constant for IM decay, measured at 24 degrees C, was strongly dependent on membrane potential, ranging from 330 ms at \u201040 mV to 12 ms at \u2010100 mV. 5. Bath application of carbachol (0.5\u201040 microM) inhibited IM, as evidenced by the reduction or elimination of the slow inward M\u2010current relaxations evoked during hyperpolarizing steps from a holding potential of \u201040 mV. The outward rectification of the steady\u2010state I\u2010V relationship at membrane potentials positive to \u201070 mV was also largely eliminated. The inhibition of IM by carbachol was dose dependent and antagonized by atropine. 6. Carbachol produced an inward current shift at a holding potential of \u201040 mV that was only partially attributable to inhibition of IM. An inward current shift was also produced by carbachol at membrane potentials negative to \u201070 mV, where IM is inactive. These effects were dose dependent and antagonized by atropine. They were attributed to the muscarinic inhibition of a voltage\u2010insensitive potassium leak conductance (ILeak). 7. In most cells, carbachol reduced the slope of the instantaneous I\u2010V relationship obtained from a holding potential of \u201070 mV so that it crossed the control I\u2010V plot at the reversal potential for ILeak. This was found to be \u2010108 mV in 3.5 mM K+ saline, shifting to \u201066 mV in 15 mM K+ saline.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019366","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T15:20:54Z","timestamp":1418829654000},"page":"93-114","source":"Crossref","is-referenced-by-count":60,"title":["Muscarinic inhibition of M\u2010current and a potassium leak conductance in neurones of the rat basolateral amygdala."],"prefix":"10.1113","volume":"457","author":[{"given":"M D","family":"Womble","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H C","family":"Moises","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1992,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019366","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T10:00:04Z","timestamp":1698055204000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,11]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019366"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019366","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11]]}}}