{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T01:21:41Z","timestamp":1698456101795},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1992,11,1]],"date-time":"1992-11-01T00:00:00Z","timestamp":720576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1992,11]]},"abstract":"<jats:p>1. Conventional whole\u2010cell voltage\u2010clamp technique was used to study the electrophysiological and pharmacological properties of voltage\u2010dependent Na+, K+ and Ca2+ channels in parasympathetic neurones enzymatically dissociated from the paratracheal ganglia of rat trachea. The voltage\u2010dependent Na+, K+ and Ca2+ currents (INa, IK and ICa) were separated by the use of ion subtraction and pharmacological treatments. 2. INa was activated by a step depolarization more positive than \u201050 mV and fully activated at positive potentials more than +10 mV. The inactivation phase of INa consisted of fast and slow exponential components (tau if and tau is, respectively). The tau if and tau is were voltage dependent and decreased with a more positive step pulse. 3. The time course for recovery of INa from the complete inactivation exhibited two exponential processes. 4. The reversal potential of INa was equal to the Na+ equilibrium potential (ENa) and resembled a simple Na+ electrode depending only on external Na+ concentration. 5. Tetrodotoxin (TTX) reduced INa without affecting the current kinetics in a concentration\u2010dependent manner, and the concentration of half\u2010maximal inhibition (IC50) was 6 x 10(\u20109) M. There was no TTX\u2010resistant component of INa in any of the cells tested. 6. Scorpion toxin increased the peak amplitude of INa and prolonged the inactivation phase in a time\u2010 and concentration\u2010dependent manner. In the presence of toxin, both tau is and the fractional contribution of the slow current component to total INa increased concentration dependently. 7. High\u2010threshold (L\u2010type) ICa was activated by a step depolarization more positive than \u201030 mV and reached a peak at near 0 mV in the external solution with 2.5 mM Ca2+. The current was inactivated to only a small extent (&lt; 10%) during 100 ms of depolarizing step pulse. There was no low\u2010threshold (T\u2010type) ICa in this preparation. 8. The maximum ICa in individual current\u2010voltage (I\u2010V) relationships was saturated by an increase in extracellular Ca2+ concentration ([Ca2+]o). The I\u2010V relationships were also shifted along the voltage axis to the more positive potential with increasing [Ca2+]o. 9. The inactivation process of the L\u2010type ICa was dependent on Ca2+ influxes (ICa\u2010dependent inactivation). 10. Relative maximum peak currents of divalent cations passing through the L\u2010type Ca2+ channels were in the order of IBa &gt; ICa &gt; ISr. 11. Organic and inorganic Ca2+ antagonists blocked the ICa in a concentration\u2010dependent manner.(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1992.sp019396","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T15:20:54Z","timestamp":1418829654000},"page":"591-610","source":"Crossref","is-referenced-by-count":18,"title":["Voltage\u2010dependent ionic currents in dissociated paratracheal ganglion cells of the rat."],"prefix":"10.1113","volume":"457","author":[{"given":"K","family":"Aibara","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Ebihara","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N","family":"Akaike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1992,11]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1992.sp019396","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1992.sp019396","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T09:59:22Z","timestamp":1698055162000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1992.sp019396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1992,11]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1992,11]]}},"alternative-id":["10.1113\/jphysiol.1992.sp019396"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1992.sp019396","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1992,11]]}}}