{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T13:52:10Z","timestamp":1747749130961},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1993,1,1]],"date-time":"1993-01-01T00:00:00Z","timestamp":725846400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1993,1]]},"abstract":"<jats:p>1. Regulation of glycogenolysis in frog skeletal muscle at rest and following contraction was studied by measuring the concentration of phosphate\u2010containing metabolites and the intracellular pH (pHi) in CN\u2010treated muscles, in which oxidative phosphorylation was inhibited by NaCN, using the 31P nuclear magnetic resonance (NMR) technique. 2. When CN\u2010treated muscles were kept at rest, the phosphocreatine (PCr) concentration very slowly decreased with time with a corresponding increase of the inorganic phosphate (Pi) concentration, while the ATP concentration remained unchanged. The pHi changed in the alkaline direction for the first 3 h, and then started to change in the acidic direction. 3. When CN\u2010treated muscles were tetanized for 10 s, the PCr concentration decreased with a corresponding increase of the Pi concentration and acidification of pHi, while the ATP concentration remained unchanged. 4. When CN\u2010treated muscles were tetanized repeatedly (each for 2 s) at constant intervals, the pHi changed in the alkaline direction following the first and the second tetani, and then changed in the acidic direction following the subsequent tetani, indicating that the consumed ATP is first replenished by the Lohmann reaction, while glycogenolysis starts only when the total amount of contractile activity exceeds a critical value. 5. Irrespective of whether CN\u2010treated muscles were kept at rest or tetanized repeatedly, the Pi concentration increased to about 8 mM (mmol\/kg wet muscle) when glycogenolysis started, suggesting that the onset of glycogenolysis in CN\u2010treated muscles is regulated by the Pi concentration. 6. The \u2018internal\u2019 buffering power of muscle cytosol was estimated to be 35 mM H+\/pH unit in anaerobic muscles and 25 mM H+\/pH unit in CN\u2010 and iodoacetic acid (IAA)\u2010treated muscles. The \u2018internal\u2019 buffering power contains a contribution due to flux of carbon dioxide and lactic acid across the cell membrane. Evidence indicated that lactic acid flux is small.<\/jats:p>","DOI":"10.1113\/jphysiol.1993.sp019471","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T14:59:45Z","timestamp":1418828385000},"page":"273-286","source":"Crossref","is-referenced-by-count":9,"title":["31P nuclear magnetic resonance studies on the glycogenolysis regulation in resting and contracting frog skeletal muscle."],"prefix":"10.1113","volume":"460","author":[{"given":"T","family":"Yamada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K","family":"Kikuchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Sugi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1993,1]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1993.sp019471","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1993.sp019471","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T15:00:13Z","timestamp":1698246013000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1993.sp019471"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,1]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,1]]}},"alternative-id":["10.1113\/jphysiol.1993.sp019471"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1993.sp019471","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,1]]}}}