{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T11:26:14Z","timestamp":1698405974096},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1993,2,1]],"date-time":"1993-02-01T00:00:00Z","timestamp":728524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1993,2]]},"abstract":"<jats:p>1. The rate of energy dissipation (E) in isolated, superfused soleus muscles from young rats was continuously measured under normosmotic and 100\u2010mosM hyperosmotic conditions. The substantial increase of E with respect to basal level in hyperosmolarity (excess E), which is entirely dependent on the presence of extracellular sodium, was largely prevented or inhibited by bumetanide, a potent inhibitor of Na(+)\u2010Cl\u2010 co\u2010transport system, or by the removal of chloride from the superfusate (isethionate substitution). Bumetanide or the removal of chloride also acutely decreased basal E, by about 7%. 2. Bumetanide almost entirely suppressed the major, Ca(2+)\u2010dependent part of excess E in hyperosmolarity, as well as the concomitant increase of 45Ca2+ efflux and small increase in resting muscle tension; in contrast, the part of excess E associated with stimulation of Na(+)\u2010H+ exchange in hyperosmolarity was left unmodified. 3. Reduction of 22Na+ influx by bumetanide was more marked in hyperosmolarity than under control conditions, although stimulation of total 22Na+ influx by a 100\u2010mosM stress was not statistically significant. Inhibition of Ca2+ release into the sarcoplasm using dantrolene sodium did not prevent the stimulation of bumetanide\u2010sensitive 22Na+ influx, but rather increased it about fourfold. 4. It is concluded that the largest part of excess E in hyperosmolarity, which is Ca(2+)\u2010dependent energy expenditure, is suppressed when steady\u2010state stimulation of a Na(+)\u2010Cl\u2010 co\u2010transport system is inhibited either directly by bumetanide or the removal of extracellular chloride, or indirectly by the blocking of active Na(+)\u2010K+ transport. How the stimulation of Na(+)\u2010Cl\u2010 co\u2010transport, by as little as 1 nmol s\u20101 (g wet muscle weight)\u20101 during a 100\u2010mosM stress, enhances Ca(2+)\u2010dependent heat by as much as 2.5 mW (g wet muscle weight)\u20101 remains to be clarified.<\/jats:p>","DOI":"10.1113\/jphysiol.1993.sp019536","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T14:51:55Z","timestamp":1418827915000},"page":"689-703","source":"Crossref","is-referenced-by-count":8,"title":["Ca(2+)\u2010dependent heat production by rat skeletal muscle in hypertonic media depends on Na(+)\u2010Cl\u2010 co\u2010transport stimulation."],"prefix":"10.1113","volume":"461","author":[{"given":"A","family":"Chinet","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1993,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1993.sp019536","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1993.sp019536","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T05:42:36Z","timestamp":1698212556000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1993.sp019536"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,2]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,2]]}},"alternative-id":["10.1113\/jphysiol.1993.sp019536"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1993.sp019536","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,2]]}}}