{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,24]],"date-time":"2025-10-24T08:07:00Z","timestamp":1761293220835},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1993,5,1]],"date-time":"1993-05-01T00:00:00Z","timestamp":736214400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1993,5]]},"abstract":"<jats:p>1. The kinetics and sensitivity of the Ca(2+)\u2010dependent inactivation of calcium current (ICa) were examined in intact cell bodies from the abdominal ganglion of Aplysia californica under two\u2010electrode voltage clamp. 2. Rapid changes in the level of intracellular free calcium ([Ca2+]i) were generated at the cell surface by photolytic release of Ca2+ (nitr\u20105 and dimethoxy nitrophen) or Ca2+ buffer (diazo\u20104). 3. Diazo\u20104 increased ICa by 10\u201015% and slowed the rate of ICa decay when photolysed before a test pulse or between a prepulse and a test pulse. The predominant effect of further light flashes was to increase the amount of non\u2010inactivating current (I infinity) remaining at the end of long (&gt; 1 s) depolarizing pulses. 4. A rapid increase in [Ca2+]i buffering during ICa inactivation did not cause a rapid recovery of current but merely reduced the rate and extent of subsequent inactivation. This effect was not seen when Ba2+ was the charge carrier. 5. Photolytic release of Ca2+ from nitr\u20105 produced estimated Ca2+ jumps of 3\u20104 microM at the front surface of the cell but failed to augment inactivation either before or during ICa. In contrast, photolysis of DM\u2010nitrophen 10\u201090 ms before the test pulse decreased peak ICa by about 30%. A flash given during ICa rapidly blocked 41 +\/\u2010 3% of peak current with a time constant of 3\u20104 ms at 17 degrees C. Similar results were seen with the barium current (IBa). 6. Microinjection of the potent phosphatase inhibitor microcystin\u2010LR (5 microM) had variable effects on ICa inactivation and augmented the cyclic AMP\u2010induced depression of the delayed rectifier (IK(V) by forskolin (100 microM) and 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX; 200 microM). 7. Full recovery from inactivation measured in two\u2010pulse experiments took at least 20 s. This slow recovery process was unaffected by increases in intracellular cyclic AMP elicited by direct injection or by bath application of forskolin and IBMX. It was also unaffected by decreases in cyclic AMP induced by injecting 2',5'\u2010dideoxyadenosine (1 mM) or bath application of the Rp isomer of cyclic adenosine 3',5'\u2010monophosphothioate (Rp\u2010cAMPS; 200 microM). 8. A 'shell\u2019 model relating submembrane Ca2+ to inactivation was inconsistent with the experimental results since it greatly overestimated the effects of diazo\u20104 and predicted significant inactivation by nitr\u20105 photolysis. 9. A model linearly relating [Ca2+]i in a single Ca2+ channel \u2018domain\u2019 to inactivation more closely matched the experimental results with diazo\u20104 and DM\u2010(ABSTRACT TRUNCATED AT 400 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1993.sp019648","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T14:29:38Z","timestamp":1418826578000},"page":"501-528","source":"Crossref","is-referenced-by-count":23,"title":["Ca(2+)\u2010dependent inactivation of Ca2+ current in Aplysia neurons: kinetic studies using photolabile Ca2+ chelators."],"prefix":"10.1113","volume":"464","author":[{"given":"M W","family":"Fryer","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R S","family":"Zucker","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1993,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1993.sp019648","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1993.sp019648","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T22:55:00Z","timestamp":1698274500000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1993.sp019648"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,5]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,5]]}},"alternative-id":["10.1113\/jphysiol.1993.sp019648"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1993.sp019648","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,5]]}}}