{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,7,26]],"date-time":"2024-07-26T06:37:04Z","timestamp":1721975824127},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1993,9,1]],"date-time":"1993-09-01T00:00:00Z","timestamp":746841600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1993,9]]},"abstract":"<jats:p>1. In this study, the mechanisms involved in the release of neurotensin\u2010like immunoreactivity (NTLI) by glucose were investigated with the isolated, vascularly perfused rat jejunoileum preparation. 2. Luminal infusion of glucose (1\u2010250 mM) produced a sharp and sustained release of NTLI in the intestinal venous effluent. The first significant response was observed with 5 mM glucose and the release reached a maximum under 250 mM glucose with a plateau secretion at 500% of basal. 3. There was no significant difference in the ability of galactose and 3\u2010O\u2010methylglucose to release NTLI when compared to glucose, but alpha\u2010methylglucose, mannose, 2\u2010deoxyglucose and fructose did not stimulate NTLI release. 4. Luminal infusion of 5 mM phloridzin reduced the glucose\u2010induced release of NTLI by 90%. Intra\u2010arterial infusion of glucose (25 mM) or of phloretin (20 microM) had no significant effect on the glucose\u2010evoked NTLI secretion. 5. Intra\u2010arterial infusion of ouabain (1 mM) produced a dramatic increase (at about 1500% of basal) in portal NTLI although it drastically reduced intestinal absorption of glucose. 6. Intra\u2010arterial infusion of tetrodotoxin (1 microM), atropine (10 microM), verapamil (50 microM) or nifedipine (50 microM) did not modify the glucose\u2010induced NTLI secretion. 7. Intra\u2010arterial infusion of forskolin (2\u201020 microM) evoked a prompt and well\u2010sustained secretion of NTLI which was increased to a mean value of 800% of basal with the highest dose tested. 3\u2010Isobutyl\u20101\u2010methylxanthine (IBMX, 10\u2010100 microM) also stimulated the secretion of NTLI (maximal increase at 725% of basal at 100 microM). In contrast, intra\u2010arterial infusion of 4\u2010beta\u2010phorbol 12\u2010myristate, 13\u2010acetate (PMA, 0.05\u20100.5 microM) had no effect on NTLI release. 8. IBMX (10\u2010100 microM) synergistically enhanced NTLI responses induced by 250 mM glucose; the integrated response of NTLI release was 3\u2010 to 5\u2010fold higher than the sum of individual responses produced by the same stimulants given separately. 9. It is concluded that the carbohydrate\u2010induced NTLI release is related to the active, sodium\u2010dependent hexose transport, but not to the carbohydrate catabolic pathway. Furthermore, the intramural nerves and L\u2010type calcium channels are not involved in the glucose\u2010induced NTLI secretion. Finally, the secretory activity of the intestinal N cell seems to be mainly stimulated through a cAMP\u2010dependent pathway.<\/jats:p>","DOI":"10.1113\/jphysiol.1993.sp019841","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T13:57:38Z","timestamp":1418824658000},"page":"753-765","source":"Crossref","is-referenced-by-count":9,"title":["Functional coupling between the active transport of glucose and the secretion of intestinal neurotensin in rats."],"prefix":"10.1113","volume":"469","author":[{"given":"T","family":"Dakka","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J C","family":"Cuber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J A","family":"Chayvialle","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1993,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1993.sp019841","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1993.sp019841","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T10:21:41Z","timestamp":1698229301000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1993.sp019841"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1993,9]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1993,9]]}},"alternative-id":["10.1113\/jphysiol.1993.sp019841"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1993.sp019841","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1993,9]]}}}