{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T20:31:33Z","timestamp":1790973093242,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1994,2,1]],"date-time":"1994-02-01T00:00:00Z","timestamp":760060800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1994,2]]},"abstract":"<jats:p>1. The contractile activation properties of human isolated bronchial smooth muscle were investigated using chemically (beta\u2010escin) skinned strips. 2. Concentration\u2010dependent contractions were induced by free ion concentrations of Ca2+ (0.5\u20103 microM), Sr2+ (2\u2010200 microM) and Ba2+ (50\u20101000 microM). The resulting \u2010log[cation]\u2010tension relationships were fitted by sigmoidal curves with EC50 values (cation concentration required to produce half\u2010maximal tension) and co\u2010operativity factors (Hill coefficient, nH) of, respectively, 0.25 microM and 3.4 for Ca2+, 12 microM and 2.64 for Sr2+ and 100 microM and 1.73 for Ba2+. Maximal responses to Sr2+ and Ba2+ were 125.5 +\/\u2010 15.4 and 96 +\/\u2010 8.1% (n = 5) respectively of the maximum tension induced by Ca2+. 3. Trifluoperazine (5\u2010100 microM), cyclic AMP (50\u2010300 microM) and cyclic GMP (50\u2010100 microM) each antagonized Ca2+ in a concentration\u2010dependent manner. On the other hand, okadaic acid (OA, 0.2\u20101 microM) potentiated Ca2+ and increased the maximum response to Ca2+ (+25 +\/\u2010 5.4%, n = 5, for 1 microM OA). 4. This study has demonstrated the high Ca2+ sensitivity of the activation mechanism of human isolated bronchial smooth muscle. It also suggests that control of the contractile machinery in the human bronchus involves processes of phosphorylation and dephosphorylation. The beta\u2010escin\u2010treated human bronchus may be a useful model for investigating the cellular basis of some pathophysiological processes such as bronchial hyper\u2010responsiveness.<\/jats:p>","DOI":"10.1113\/jphysiol.1994.sp020034","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T13:13:58Z","timestamp":1418822038000},"page":"433-438","source":"Crossref","is-referenced-by-count":28,"title":["Activation properties of chemically skinned fibres from human isolated bronchial smooth muscle."],"prefix":"10.1113","volume":"474","author":[{"given":"J P","family":"Savineau","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R","family":"Marthan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1994,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1994.sp020034","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1994.sp020034","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T00:22:35Z","timestamp":1698106955000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1994.sp020034"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,2]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,2]]}},"alternative-id":["10.1113\/jphysiol.1994.sp020034"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1994.sp020034","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,2]]}}}