{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,9]],"date-time":"2026-01-09T20:32:36Z","timestamp":1767990756185,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1994,8,1]],"date-time":"1994-08-01T00:00:00Z","timestamp":775699200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1994,8]]},"abstract":"<jats:p>1. The occurrence of extracellular alkaline transients during excitatory synaptic transmission suggests that the NMDA receptor H(+)\u2010modulatory site may have a physiological role. Here we amplify these pH shifts using benzolamide (a carbonic anhydrase inhibitor) and describe concomitant effects on EPSCs in whole\u2010cell clamped CA1 neurones in rat hippocampal slices. 2. In CO2\u2010HCO3(\u2010)\u2010buffered media, benzolamide increased the time to 50% decay (t50) of the EPSCs by 78 +\/\u2010 14% (P &lt; 0.01, n = 10). This occurred simultaneously with amplification of the extracellular alkaline shift (154 +\/\u2010 14%). 3. In CO2\u2010HCO3(\u2010)\u2010buffered media containing DL\u20102\u2010amino\u20105\u2010phosphonovalerate (APV), the EPSC t50 was unaltered by benzolamide, while the extracellular alkaline shifts were increased (111 +\/\u2010 23%, n = 8). 4. In Hepes\u2010buffered media, neither the EPSC t50 nor the extracellular alkaline shift was altered by benzolamide (n = 9). 5. These data demonstrate that NMDA receptor activity is dependent on the buffering kinetics of the brain extracellular space. The results suggest that endogenous pH shifts can modulate NMDA receptor function in a physiologically relevant time frame.<\/jats:p>","DOI":"10.1113\/jphysiol.1994.sp020258","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T12:22:39Z","timestamp":1418818959000},"page":"373-378","source":"Crossref","is-referenced-by-count":64,"title":["Endogenous H+ modulation of NMDA receptor\u2010mediated EPSCs revealed by carbonic anhydrase inhibition in rat hippocampus."],"prefix":"10.1113","volume":"478","author":[{"given":"J A","family":"Gottfried","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Chesler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,8]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1994.sp020258","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1994.sp020258","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T17:22:58Z","timestamp":1698168178000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1994.sp020258"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1994,8]]}},"alternative-id":["10.1113\/jphysiol.1994.sp020258"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1994.sp020258","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8]]}}}