{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,6,17]],"date-time":"2024-06-17T19:59:43Z","timestamp":1718654383655},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1994,8,15]],"date-time":"1994-08-15T00:00:00Z","timestamp":776908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1994,8,15]]},"abstract":"<jats:p>1. Non\u2010linear membrane ionic current was studied in highly stretched cut frog twitch fibres in a double Vaseline\u2010gap voltage clamp chamber, with the internal solution containing 0.1 mM EGTA and the external solution containing Cl\u2010 as the major anion. After the Na+ currents was abolished by TTX in the external solution and the K+ currents were suppressed by external TEA+ and Rb+ and internal Cs+, a delayed outward ionic current with a time course similar to that of the delayed rectifier current was observed during depolarization. 2. The delayed outward ionic current was resistant to 1 mM 3,4\u2010diaminopyridine (3,4\u2010DAP) in the external solution and was unaltered when a fraction of the internal Cs+ was replaced by K+ or Na+, suggesting that the current was not carried by cations flowing through the delayed rectifiers. 3. The delayed outward ionic current was greatly reduced by replacing the external Cl\u2010 with CH3SO3\u2010,SO4(2\u2010), glutamate or gluconate, indicating strongly that the current was carried by Cl\u2010 flowing through anion channels. The current was also suppressed by 1 mM external 9\u2010anthracenecarboxylic acid (9\u2010ACA). 4. The delayed outward ionic current was reduced by blockers of calcium\u2010dependent Cl\u2010 channels, such as SITS and frusemide (furosemide), in a dose\u2010 and voltage\u2010dependent manner and by increasing intracellular [EGTA] to 20 mM, suggesting that part of the Cl\u2010 current in the muscle fibres could be calcium dependent. 5. The total Cl\u2010 current could be dissected into calcium\u2010dependent and calcium\u2010independent components. Each component accounted for roughly half of the total Cl\u2010 current. The maximum slope conductance of the calcium\u2010dependent Cl\u2010 channels was 60.9 +\/\u2010 6.0 microS microF\u20101 (mean +\/\u2010 S.E.M., n = 4).<\/jats:p>","DOI":"10.1113\/jphysiol.1994.sp020281","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T12:01:30Z","timestamp":1418817690000},"page":"109-125","source":"Crossref","is-referenced-by-count":7,"title":["Origin of delayed outward ionic current in charge movement traces from frog skeletal muscle."],"prefix":"10.1113","volume":"479","author":[{"given":"C S","family":"Hui","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W","family":"Chen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1994,8,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1994.sp020281","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1994.sp020281","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T09:18:36Z","timestamp":1698225516000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1994.sp020281"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1994,8,15]]}},"alternative-id":["10.1113\/jphysiol.1994.sp020281"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1994.sp020281","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,8,15]]}}}