{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T11:27:22Z","timestamp":1790508442864,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1994,9,1]],"date-time":"1994-09-01T00:00:00Z","timestamp":778377600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1994,9]]},"abstract":"<jats:p>1. Previous work has shown that one mechanism by which blood\u2010borne interleukin\u20101 alpha (IL\u20101) may be able to affect the central nervous system (CNS) is by direct transport into the brain across the blood\u2010brain barrier (BBB). The BBB of the brain consists of endothelial (between blood and interstitial fluid) and ependymal (between blood and cerebrospinal fluid) barriers. Which of these barriers IL\u20101 can cross has not previously been investigated. At the spinal cord, which could be the site of action for some of the effects of IL\u20101 such as analgesia, the BBB consists only of the endothelial barrier. 2. We show here that IL\u20101 labelled with 125I (I\u2010IL) is transported across the BBB of the spinal cord by a saturable system similar to the one previously described for the brain. High performance liquid chromatography (HPLC) showed that most of the material entering the spinal cord represented intact I\u2010IL. The BBB of the spinal cord was no more leaky to radioactively labelled albumin than the BBB of the brain and was not disrupted by 50 micrograms kg\u20101 of IL\u20101. 3. Capillary depletion showed that most of the I\u2010IL entered the parenchymal\u2010interstitial fluid space of the spinal cord with only a modest amount being sequestered by the endothelial cells of its BBB. 4. I\u2010IL entered the cervical, thoracic and lumbar regions of the spinal cord equally well. I\u2010IL entering at the brain and diffusing caudally was estimated only to account for about 1% of the total radioactivity found in the spinal cord after i.v. injection.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1994.sp020293","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T12:04:39Z","timestamp":1418817879000},"page":"257-264","source":"Crossref","is-referenced-by-count":50,"title":["Blood\u2010borne interleukin\u20101 alpha is transported across the endothelial blood\u2010spinal cord barrier of mice."],"prefix":"10.1113","volume":"479","author":[{"given":"W A","family":"Banks","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A J","family":"Kastin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C A","family":"Ehrensing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1994,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1994.sp020293","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1994.sp020293","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,24]],"date-time":"2023-10-24T12:44:11Z","timestamp":1698151451000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1994.sp020293"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,9]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1994,9]]}},"alternative-id":["10.1113\/jphysiol.1994.sp020293"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1994.sp020293","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,9]]}}}