{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:17:00Z","timestamp":1758925020304},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1995,4,1]],"date-time":"1995-04-01T00:00:00Z","timestamp":796694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1995,4]]},"abstract":"<jats:p>1. The responses of guinea\u2010pig endocardial endothelial (EE) cells to various vasoactive substances were investigated in either the small tissue preparation or freshly isolated cells using the patch clamp technique. 2. The mean resting potential of the EE cell was \u201044 mV in the small tissue preparation, and applications of ATP, ADP, AMP, adenosine, histamine and substance P induced transient hyperpolarizations of \u201022, \u201021, \u20109, \u201010, \u201023 and \u201015 mV, respectively. The membrane potential of EE cells failed to respond to acetylcholine, bradykinin, thrombin, atrial natriuretic peptide, vasopressin and serotonin. 3. The whole\u2010cell voltage clamp of dissociated cells revealed a transient increase of K+ conductance underlying the ATP and histamine responses. The agonist\u2010induced current showed no time\u2010dependent change during voltage steps. The response was showed no time\u2010dependent change during voltage steps. The response was prevented by adding 10 mM EGTA to the pipette solution. 4. In the cell\u2010attached single channel recordings, ATP induced transient K+ channel activities having a slope conductance of 34 pS. In inside\u2010out patches, similar K+ channels were activated by applying Ca2+ of more than 0.1 microM. 5. These findings are consistent with the idea that the Ca(2+)\u2010dependent K+ channel is involved in the hyperpolarizing response of EE cells, as described in vascular endothelial cells.<\/jats:p>","DOI":"10.1113\/jphysiol.1995.sp020645","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T10:40:08Z","timestamp":1418812808000},"page":"25-40","source":"Crossref","is-referenced-by-count":13,"title":["Hyperpolarization induced by vasoactive substances in intact guinea\u2010pig endocardial endothelial cells."],"prefix":"10.1113","volume":"484","author":[{"given":"K","family":"Manabe","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Ito","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Matsuda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A","family":"Noma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1995.sp020645","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1995.sp020645","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T15:31:03Z","timestamp":1698247863000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1995.sp020645"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,4]]}},"alternative-id":["10.1113\/jphysiol.1995.sp020645"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1995.sp020645","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,4]]}}}