{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T12:42:37Z","timestamp":1789994557747,"version":"4.0.1"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1995,4,1]],"date-time":"1995-04-01T00:00:00Z","timestamp":796694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1995,4]]},"abstract":"<jats:p>1. Conventional and ion\u2010selective double\u2010barrelled microelectrodes were used in an in vitro preparation of bovine retinal pigment epithelium (RPE)\u2010choroid to measure the changes in membrane voltage, resistance and intracellular Cl\u2010 activity (aCli) produced by small, physiological changes in extracellular potassium concentration ([K+]o). These apical [K+]o changes approximate those produced in the extracellular (subretinal) space between the photoreceptors and the RPE following transitions between light and dark. 2. Changing apical [K+]o from 5 to 2 mM in vitro elicited membrane voltage responses with three distinct phases. The first phase was generated by an apical membrane hyperpolarization, followed by a (delayed) basolateral membrane hyperpolarization (DBMH); the third phase was an apical membrane depolarization. The present experiments focus on the membrane and cellular mechanisms that generate phase 2 of the response, the DBMH. 3. The DBMH was abolished in the presence of apical bumetanide (100 microM); this response was completely restored after bumetanide removal. 4. Reducing apical [K+]o, adding apical bumetanide (500 mM), or removing apical Cl\u2010 decreased aCli by 25 +\/\u2010 6 (n = 8), 28 +\/\u2010 1 (n = 2) and 26 +\/\u2010 5 mM (n = 3), respectively; adding 100 microM apical bumetanide decreased aCli by 12 +\/\u2010 2 mM (n = 3). Adding apical bumetanide or removing apical bath Cl\u2010 hyperpolarized the basolateral membrane and decreased the apparent basolateral membrane conductance (GB). 5. DIDS (4,4'\u2010diisothiocyanostilbene\u20102,2'\u2010disulphonic acid) blocked the RPE basolateral membrane Cl\u2010 conductance and inhibited the DBMH and the basolateral membrane hyperpolarization produced by apical bumetanide addition or by removal of apical Cl\u2010o. The present results show that the DBMH is caused by delta[K]o\u2010induced inhibition of the apical membrane Na(+)\u2010K(+)\u20102Cl\u2010 cotransporter; the subsequent decrease in aCli generated a hyperpolarization at the basolateral membrane Cl\u2010 channel.<\/jats:p>","DOI":"10.1113\/jphysiol.1995.sp020647","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T10:40:08Z","timestamp":1418812808000},"page":"53-67","source":"Crossref","is-referenced-by-count":30,"title":["The delayed basolateral membrane hyperpolarization of the bovine retinal pigment epithelium: mechanism of generation."],"prefix":"10.1113","volume":"484","author":[{"given":"S","family":"Bialek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D P","family":"Joseph","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S S","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1995,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1995.sp020647","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1995.sp020647","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T15:31:25Z","timestamp":1698247885000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1995.sp020647"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,4]]}},"alternative-id":["10.1113\/jphysiol.1995.sp020647"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1995.sp020647","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,4]]}}}