{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T15:17:27Z","timestamp":1761059847802},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1995,4,1]],"date-time":"1995-04-01T00:00:00Z","timestamp":796694400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1995,4]]},"abstract":"<jats:p>1. Chicken enterocytes present a Na(+)\u2010independent proton transport mechanism involved in pHi recovery from an acid load. In the current study the nature of this proton transport system is investigated. 2. The pHi of acid\u2010loaded cells increased when transferred to Na(+)\u2010free, pH 7.4 buffers, both at 6 and 65 mM extracellular potassium concentration. Addition of nigericin accelerated the rate of cell alkalinization. 3. When acid\u2010loaded cells were transferred to a Na(+)\u2010free, pH 6.5 buffer, the cells acidified further, regardless of the extracellular potassium concentration. The addition of nigericin increased the rate of acidification at 6 mM K+ but produced an alkalinization at 65 mM K+. 4. The rate of the Na(+)\u2010independent regulatory cell alkalinization was inhibited by SCH 28080, DCCD, NBD\u2010Cl, rotenone or Zn2+. Addition of valinomycin reversed the inhibition induced by SCH 28080, DCCD and NBD\u2010Cl but not that induced by Zn2+ or rotenone. Zn2+ inhibition was abolished by the metal chelator DTPA. 5. Cytosolic acidification increased the rate of Na(+)\u2010independent regulatory cell alkalinization. 6. The results suggest that the Na(+)\u2010independent proton transport system is a Zn(2+)\u2010sensitive proton\u2010conducting pathway which is regulated by the cytosolic proton concentration.<\/jats:p>","DOI":"10.1113\/jphysiol.1995.sp020655","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T10:40:08Z","timestamp":1418812808000},"page":"165-172","source":"Crossref","is-referenced-by-count":9,"title":["Proton conductance and intracellular pH recovery from an acid load in chicken enterocytes."],"prefix":"10.1113","volume":"484","author":[{"given":"M J","family":"Peral","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A A","family":"Ilund\u00e1in","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,4]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1995.sp020655","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1995.sp020655","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,25]],"date-time":"2023-10-25T15:31:04Z","timestamp":1698247864000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1995.sp020655"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,4]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,4]]}},"alternative-id":["10.1113\/jphysiol.1995.sp020655"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1995.sp020655","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,4]]}}}