{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T22:45:59Z","timestamp":1778021159001,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1995,9,1]],"date-time":"1995-09-01T00:00:00Z","timestamp":809913600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1995,9]]},"abstract":"<jats:p>1. The whole\u2010cell configuration of the patch\u2010clamp technique was used to analyse currents induced by glycine in chick ciliary ganglion neurons freshly dissociated from 14\u2010 to 15\u2010day\u2010old embryos. 2. Application of glycine to cells voltage\u2010clamped at \u201060 mV induced inward currents in all neurons tested. Dose\u2010response curves yielded an EC50 of about 50 microM. Similar responses were elicited by beta\u2010alanine and taurine though higher concentrations were required. 3. Strychnine reversibly inhibited the glycine\u2010induced responses. The effect was dose dependent with a half\u2010maximal effect being obtained with 20 nM strychnine. 4. Glycine\u2010induced currents were inhibited by 100 microM Zn2+. The inhibition had slow rates of onset and recovery, in contrast to Zn2+ inhibition of GABA responses. Both bicuculline and d\u2010tubocurarine inhibited glycine responses in a dose\u2010dependent manner. 5. The steady\u2010state I\u2010V curve for glycine\u2010induced currents was linear over the range \u201060 to +60 mV, but showed an outward rectification at very hyperpolarized membrane potentials. The reversal potential of glycine\u2010induced currents shifted with changes in intracellular chloride concentration in a manner expected for chloride\u2010selective channels. 6. Expression of functional glycine receptors during development was examined at embryonic day 8 (E8), 11, 14, and 18. The mean peak current was about 60\u2010fold larger at E14 than at E8 and vastly exceeded changes in cell size. During the same period, responses to GABA increased only 2\u2010fold in amplitude and correlated with changes in cell size.(ABSTRACT TRUNCATED AT 250 WORDS)<\/jats:p>","DOI":"10.1113\/jphysiol.1995.sp020888","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T10:01:58Z","timestamp":1418810518000},"page":"395-405","source":"Crossref","is-referenced-by-count":25,"title":["Patch\u2010clamp analysis of glycine\u2010induced currents in chick ciliary ganglion neurons."],"prefix":"10.1113","volume":"487","author":[{"given":"Z W","family":"Zhang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D K","family":"Berg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1995.sp020888","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1995.sp020888","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T04:56:36Z","timestamp":1698296196000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1995.sp020888"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,9]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1995,9]]}},"alternative-id":["10.1113\/jphysiol.1995.sp020888"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1995.sp020888","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,9]]}}}