{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,26]],"date-time":"2025-09-26T22:16:25Z","timestamp":1758924985115},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1995,11,15]],"date-time":"1995-11-15T00:00:00Z","timestamp":816393600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1995,11,15]]},"abstract":"<jats:p>1. Intracellular recordings were used to study the role of metabotropic glutamate receptors (mGluRs) in modulating GABA\u2010mediated giant depolarizing potentials (GDPs) in immature rat hippocampal CA3 neurones. 2. The mGluR antagonist (RS)\u2010alpha\u2010methyl\u20104\u2010carboxyphenylglycine (MCPG, 1 mM) reduced the frequency of GDPs. The broad\u2010spectrum ionotropic glutamate receptor antagonist kynurenic acid (1 mM) blocked GDPs. 3. In the presence of kynurenic acid, both tetanic stimulation of the hilus or bath application of quisqualic acid (1 microM) and trans\u20101\u2010aminocyclopentane\u20101,3\u2010dicarboxylic acid (t\u2010ACPD, 20 microM) induced the appearance of GDPs. These effects were antagonized by MCPG (1 mM) or L(+)\u20102\u2010amino\u20103\u2010phosphonopropionic acid (L\u2010AP3) and blocked by bicuculline (10 microM). 4. 8\u2010Bromo\u2010cAMP (8\u2010Br\u2010cAMP, 0.3 mM), 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX, 200 microM) or forskolin (30 microM) mimicked the effects of mGluR agonists on GDPs. The forskolin analogue 1,9\u2010dideoxyforskolin (30 microM), which does not activate adenylate cyclase, was ineffective. 5. Incubation of slices in the presence of the protein kinase A inhibitor Rp\u2010adenosine 3',5'\u2010cyclic monophosphothioate triethylamine (Rp\u2010cAMPS) (500 microM) or superfusion of Rp\u2010cAMPS (20 microM) prevented the effects of forskolin or t\u2010ACPD on GDPs. In the presence of kynurenic acid, the protein kinase C activator, phorbol 12,13\u2010diacetate (2 microM) induced the appearance of GDPs. This effect was prevented by staurosporine (1 microM). However, staurosporine (1\u20103 microM) did not modify the effects of t\u2010ACPD on GDPs. 6. It is suggested that, during development, mGluRs enhance the synchronous release of GABA, responsible for GDPs, through cAMP\u2010dependent protein kinase.<\/jats:p>","DOI":"10.1113\/jphysiol.1995.sp021035","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T09:27:04Z","timestamp":1418808424000},"page":"115-125","source":"Crossref","is-referenced-by-count":20,"title":["Cyclic AMP\u2010dependent modulation of giant depolarizing potentials by metabotropic glutamate receptors in the rat hippocampus."],"prefix":"10.1113","volume":"489","author":[{"given":"F","family":"Strata","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Sciancalepore","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E","family":"Cherubini","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1995,11,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1995.sp021035","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1995.sp021035","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T02:32:44Z","timestamp":1698287564000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1995.sp021035"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,11,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1995,11,15]]}},"alternative-id":["10.1113\/jphysiol.1995.sp021035"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1995.sp021035","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,11,15]]}}}