{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T15:46:08Z","timestamp":1698421568790},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1996,2,15]],"date-time":"1996-02-15T00:00:00Z","timestamp":824342400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,2,15]]},"abstract":"<jats:p>1. Chloride accumulation into rat saphenous arterial smooth muscle has been examined using chloride\u2010sensitive microelectrodes, to assess the contribution of Na(+)\u2010Cl\u2010cotransport. 2. Bumetanide (10 microM) produced a fall in intracellular chloride ([Cl\u2010]i), and a hyperpolarization of membrane potential (Em). However, [Cl\u2010]i remained above the equilibrium level predicted from the membrane potential, indicating a residual accumulation. 3. Replacement of extracellular sodium with N\u2010methyl\u2010D\u2010glucamine or choline caused a fall in [Cl\u2010]i similar to that observed with bumetanide, but the hyperpolarization of Em was larger. In Na(+)\u2010free media, bumetanide had no effect. [Cl\u2010]i remained significantly above equilibrium. 4. In the presence of bumetanide, chlorothiazide produced a further dose\u2010dependent fall in [Cl\u2010]i, and hyperpolarization of Em. However, although [Cl\u2010]i fell more than with bumetanide alone, it remained significantly above equilibrium. Metolazone was without effect at 100 microM. 5. In the presence of bumetanide, ethacrynic acid and N\u2010ethyl maleimide caused a dose\u2010dependent hyperpolarization of Em and a fall in [Cl\u2010]i to equilibrium. 6. The third inward chloride pump in rat saphenous arterial smooth muscle appears not to be a form of Na(+)\u2010Cl\u2010 cotransport. The potency series of thiazide diuretic action (acetazolamide &gt; chlorothiazide &gt; metolazone) differed significantly from that published for Na(+)\u2010Cl\u2010 cotransport, and there is no sodium dependence.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021196","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T08:42:40Z","timestamp":1418805760000},"page":"61-68","source":"Crossref","is-referenced-by-count":16,"title":["Evidence against a contribution by Na(+)\u2010Cl\u2010 cotransport to chloride accumulation in rat arterial smooth muscle."],"prefix":"10.1113","volume":"491","author":[{"given":"J P","family":"Davis","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,2,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021196","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021196","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T22:35:32Z","timestamp":1698359732000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021196"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,2,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,2,15]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021196"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021196","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,2,15]]}}}