{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T22:11:38Z","timestamp":1762899098559},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1996,3,15]],"date-time":"1996-03-15T00:00:00Z","timestamp":826848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,3,15]]},"abstract":"<jats:p>1. The whole\u2010cell patch clamp technique was used to investigate the swelling\u2010activated currents in bovine non\u2010pigmented ciliary epithelial (NPCE) cells. 2. Exposure to hypotonic solution activated a current that was blocked by 5\u2010nitro\u20102\u2010(3\u2010phenylpropylamino)benzoic acid (NPPB). The I\u2010V relationship was shifted in the direction expected for a Cl\u2010 current when the external Cl\u2010 was replaced by gluconate (permeability ratio P(gluconate)\/PCl = 0.17). The inhibition of the current evoked by voltage clamp steps of +80 mV yielded an IC50 for NPPB of 13.4 microM. 3. The current was found to be dependent on ATP. With ATP in the patch pipette the current could be repeatedly activated by exposure to hypotonic solution but when ATP was omitted the current ran down with time. 4. The development of this current was associated with visible cell swelling and inhibitors of regulatory volume decrease in these cells, e.g. tamoxifen, 4\u2010acetamido\u20104'\u2010isothiocyanatostilbene\u20102,2'\u2010disulphonic acid (SITS) and 4,4'\u2010diisothiocyanostilbene\u20102,2'\u2010disulphonic acid (DIDS), also inhibited this current. 5. The volume\u2010activated current was additionally blocked by NPPB, verapamil, quinidine and dideoxyforskolin. 6. The current was independent of external calcium and exhibited slight outward rectification and time\u2010dependent inactivation at strong depolarizing potentials. 7. Disrupting the cytoskeleton and microtubules with cytochalasin B and colchicine had no effect on the activation of the Cl\u2010 current. 8. An antibody (C219) to the MDR1 gene product, P\u2010glycoprotein, caused a functional block of the swelling\u2010activated Cl\u2010 current when added to the patch pipette. 9. Immunofluorescence studies using the monoclonal antibodies C219 and JSB\u20101 demonstrated the presence of P\u2010glycoprotein in the ciliary epithelial cells. The immunofluorescence was stronger on the non\u2010pigmented than on the pigmented cells. 10. It is concluded that swelling in NPCE cells activates a Ca(2+)\u2010independent, ATP\u2010dependent Cl\u2010 current and that the activity of this current is associated with P\u2010glycoprotein. 11. It is suggested that this Cl\u2010 current contributes to regulatory volume decrease and may participate in the secretory activity of these cells.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021254","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T08:57:36Z","timestamp":1418806656000},"page":"743-755","source":"Crossref","is-referenced-by-count":63,"title":["P\u2010glycoprotein regulates a volume\u2010activated chloride current in bovine non\u2010pigmented ciliary epithelial cells."],"prefix":"10.1113","volume":"491","author":[{"given":"J","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J J","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Koppel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T J","family":"Jacob","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,3,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021254","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021254","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T20:40:52Z","timestamp":1698352852000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021254"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,3,15]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,3,15]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021254"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021254","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,3,15]]}}}