{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:51:25Z","timestamp":1790862685361,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1996,5,1]],"date-time":"1996-05-01T00:00:00Z","timestamp":830908800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,5]]},"abstract":"<jats:p>1. Modulatory actions of serotonin (5\u2010hydroxytryptamine, 5\u2010HT) on excitatory postsynaptic currents (EPSCs) were studied with whole\u2010cell recordings from superficial dorsal horn (SDH) neurones in neonatal rat spinal cord slices. In one\u2010third of SDH neurones, 5\u2010HT induced a sustained potentiation of evoked EPSCs lasting for more than 30 min after wash\u2010out. This potentiation was often preceded by a transient suppression of EPSCs. 2. Serotonin differentially modulated the frequency of miniature EPSCs recorded in the presence of tetrodotoxin (TTX) according to the SDH neurones, producing a transient suppression, a transient facilitation or a long\u2010lasting facilitation. 3. The 5\u2010HT1A\u2010receptor agonist 8\u2010hydroxy\u20102\u2010(di\u2010n\u2010propylamino)tetralin (8\u2010OH\u2010DPAT) suppressed the amplitude of evoked EPSCs and frequency of miniature EPSCs in a reversible manner. In contrast, the 5\u2010HT2\u2010receptor agonists 1\u2010(2,5\u2010dimethoxy\u20104\u2010iodophenyl)\u20102\u2010aminopropane (DOI) and alpha\u2010methyl\u20105\u2010HT induced long\u2010lasting potentiations of EPSC amplitude and miniature EPSC frequency. 4. Neither the mean amplitude nor the kinetics of miniature EPSCs were affected by 5\u2010HT during the sustained facilitation of miniature EPSC frequency, suggesting that the facilitatory effect of 5\u2010HT was presynaptically mediated. The 5\u2010HT\u2010induced long\u2010lasting facilitation of miniature EPSC frequency was observed also in Ca(2+)\u2010free, Mg2+ solution. 5. The long\u2010lasting facilitation of evoked EPSC amplitude and miniature EPSC frequency by 5\u2010HT was mimicked by the phorbol ester, phorbol 12,13\u2010dibutyrate (PDBu), and blocked reversibly by the protein kinase C (PKC) inhibitor, calphostin C. Forskolin applied together with 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX) had no effect on the evoked EPSCs. 6. We conclude that serotonin can induce a long\u2010lasting facilitation of evoked EPSCs and spontaneous release of excitatory transmitter at SDH synapses of rat spinal cord. Our results suggest that intracellular PKC linked to the 5\u2010HT2 receptor may mediate this effect by directly activating the exocytotic machinery.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021352","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T08:34:03Z","timestamp":1418805243000},"page":"867-876","source":"Crossref","is-referenced-by-count":122,"title":["Long\u2010lasting synaptic facilitation induced by serotonin in superficial dorsal horn neurones of the rat spinal cord."],"prefix":"10.1113","volume":"492","author":[{"given":"Y","family":"Hori","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K","family":"Endo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T","family":"Takahashi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1996,5]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021352","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021352","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T19:59:53Z","timestamp":1698436793000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021352"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,5]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1996,5]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021352"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021352","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,5]]}}}