{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T09:49:57Z","timestamp":1789552197736,"version":"build-2803163510"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1996,6,1]],"date-time":"1996-06-01T00:00:00Z","timestamp":833587200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,6]]},"abstract":"<jats:p>1. We investigated the mechanisms regulating acid extrusion in cultured fetal rat hippocampal neurones loaded with 2',7'\u2010bis\u2010(2\u2010carboxyethyl)\u20105\u2010(and\u20106)\u2010carboxyfluorescein. 2. In the absence of HCO3\u2010, removal of external Na+ by substitution with N\u2010methyl\u2010D\u2010glucamine caused a sustained intracellular acidification that was not observed when Na+ was replaced by Li+, but neither steady\u2010state intracellular pH (pHi) nor the rate of pHi recovery from an imposed acid load were influenced by amiloride analogues or HOE 694, inhibitors of Na(+)\u2010H+ exchange in other cell types. In the presence of HCO3\u2010, removal of external Na+ or Cl\u2010 evoked an intracellular acidification and a 4,4'\u2010diisothiocyanatostilbene\u20102,2'\u2010disulphonic acid\u2010sensitive (DIDS\u2010sensitive) intracellular alkalinization, respectively. Applied alone, however, DIDS elicited a fall in steady\u2010state pHi at room temperature but not at 37 degrees C. The DIDS\u2010evoked fall in steady\u2010state pHi and the 0 Cl(\u2010)\u2010evoked intracellular alkalinization observed in the presence of HCO3\u2010 at room temperature were dependent on external Na+. 3. At room temperature (18\u201022 degrees C), but not at 37 degrees C, the transition from HCO3(\u2010)\u2010free to HCO3(\u2010)\u2010containing medium at a constant pHo produced a net alkalinization that was dependent on external Na+ and was inhibited by DIDS or the depletion of internal Cl\u2010. 4. Recovery of pHi from an acid load imposed in the absence of HCO3\u2010 was dependent on external Na+. Addition of HCO3\u2010 to the perfusion medium increased the rate of pHi recovery from an acid load at room temperature but not at 37 degrees C. In the presence of HCO3\u2010, DIDS slowed the rate of recovery of pHi from an acid load at both room temperature and at 37 degrees C. 5. Recovery of pHi following an imposed intracellular acidification to pH &lt; 6.5 could occur in the absence of external Na+, providing that HCO3\u2010 was present in the perfusate. This slow, Na(+)\u2010independent recovery of pHi from very low levels of intracellular pH was sensitive to DIDS. 6. The results indicate that acid extrusion in cultured fetal rat hippocampal neurones involves primarily two Na(+)\u2010dependent mechanisms, one HCO3\u2010 dependent (a HCO3(\u2010)\u2010Cl\u2010 exchanger) and the other HCO3\u2010 independent (possibly a Na(+)\u2010H+ exchanger). Although both mechanisms participate in the maintenance of steady\u2010state pHi at room temperature, only the HCO3(\u2010)\u2010independent mechanism does so at 37 degrees C.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021396","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T08:07:16Z","timestamp":1418803636000},"page":"457-470","source":"Crossref","is-referenced-by-count":65,"title":["Characterization of acid extrusion mechanisms in cultured fetal rat hippocampal neurones."],"prefix":"10.1113","volume":"493","author":[{"given":"K A","family":"Baxter","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J","family":"Church","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1996,6]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021396","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021396","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T06:01:09Z","timestamp":1698386469000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021396"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,6]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,6]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021396"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021396","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,6]]}}}