{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:21:54Z","timestamp":1778275314360,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1996,8,15]],"date-time":"1996-08-15T00:00:00Z","timestamp":840067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,8,15]]},"abstract":"<jats:p>1. Short segments of interlobular duct were microdissected from guinea\u2010pig pancreas following enzymatic digestion. After overnight culture, intracellular pH (pH1) and Na+ concentration ([Na+]i) were measured by microfluorometry in duct cells loaded with either the pH\u2010sensitive fluoroprobe 2'7'\u2010bis(2\u2010carboxyethyl)\u20105(6)\u2010carboxyfluorescein (BCECF) or the sodium\u2010binding benzofuran isophthalate (SBFI). 2. The transporters responsible for maintaining pHi above equilibrium were investigated by using the NH4Cl pulse technique to acid load the cells. In the absence of HCO3\u2010\/CO2, the recovery of pH1 was Na+ dependent, abolished by 0.2 mM amiloride and by 10 microM N\u2010methyl\u2010N\u2010isobutylamiloride and was therefore attributed to Na(+)\u2010H+ exchange. 3. In the presence of HCO3\u2010\/CO2, amiloride only partially inhibited the recovery from acid loading. The amiloride\u2010insensitive component was abolished by 0.5 mM H2DIDS and unaffected by depletion of intracellular Cl\u2010 and was therefore attributed to Na(+)\u2010HCO3\u2010 cotransport. 4. Stimulation with 10 nM secretin did not cause a significant change in pH1 despite a significant increase in HCO3\u2010 efflux. However, in the presence of secretin, addition of 0.5 mM H2DIDS caused a decline in pH1 that was three times more rapid than that obtained with 0.2 mM amiloride. 5. In secretin\u2010stimulated ducts, Na+ uptake increased when HCO3\u2010\/CO2 was added to the bath and this increase was strongly inhibited by 0.5 mM H2DIDS. 6. We conclude that Na(+)\u2010HCO3\u2010 cotransport contributes approximately 75% of the HCO3\u2010 taken up by guinea\u2010pig pancreatic duct cells during stimulation with secretin. It is proposed that electrical coupling between HCO3\u2010 efflux at the luminal membrane and electrogenic Na(+)\u2010HCO3\u2010 cotransport at the basolateral membrane explains why secretin causes little change in pH1.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021582","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T07:21:23Z","timestamp":1418800883000},"page":"169-178","source":"Crossref","is-referenced-by-count":140,"title":["Accumulation of intracellular HCO3\u2010 by Na(+)\u2010HCO3\u2010 cotransport in interlobular ducts from guinea\u2010pig pancreas."],"prefix":"10.1113","volume":"495","author":[{"given":"H","family":"Ishiguro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M C","family":"Steward","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A R","family":"Lindsay","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R M","family":"Case","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,8,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021582","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021582","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T21:04:16Z","timestamp":1698440656000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021582"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,8,15]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021582"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021582","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,8,15]]}}}