{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,8]],"date-time":"2026-05-08T21:21:52Z","timestamp":1778275312328,"version":"3.51.4"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1996,8,15]],"date-time":"1996-08-15T00:00:00Z","timestamp":840067200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,8,15]]},"abstract":"<jats:p>1. The transport of HCO3\u2010 across the luminal membrane of pancreatic duct cells was studied by monitoring the luminal pH of isolated guinea\u2010pig interlobular ducts after microinjection of an extracellular fluoroprobe, the dextran conjugate of 2'7'\u2010bis(2\u2010carboxyethyl)\u20105(6)\u2010carboxyfluorescein (BCECF\u2010dextran). Luminal Cl\u2010 concentration was also measured by microfluorometry following microinjection of the dextran conjugates of 6\u2010methoxy\u2010N\u2010(4\u2010aminoalkyl)quinolinium bromide (ABQ\u2010dextran) and Cl\u2010NERF (Cl\u2010NERF\u2010dextran). 2. When HCO3\u2010\/CO2 was admitted to the bath, a transient acidification of the duct lumen was observed, followed by a marked alkalinization. The latter was abolished when the luminal Cl\u2010 concentration was reduced to 25\u201035 mM by replacement with glucuronate and may, therefore, be attributed to Cl(\u2010)\u2010HCO3\u2010 exchange at the luminal membrane. 3. Secretin, forskolin and acetylcholine stimulated HCO3\u2010 secretion into the lumen even when the luminal Cl\u2010 concentration was reduced to approximately 7 mM. Furthermore, agonist\u2010evoked HCO3\u2010 secretion was not inhibited by luminal glibenclamide, dihydro\u20104,4'\u2010diisothiocyanostilbene\u20102,2'\u2010disulphonic acid (H2DIDS) or 5\u2010nitro\u20102\u2010(3\u2010phenylpropylamino)\u2010benzoic acid (NPPB). These observations are not easily reconciled with HCO3\u2010 transport across the luminal membrane being mediated by Cl(\u2010)\u2010HCO3\u2010 exchange in parallel with a Cl\u2010 conductance. 4. Agonist\u2010stimulated HCO3\u2010 secretion was blocked by omitting Na+ from the bath but not by addition of N\u2010methyl\u2010N\u2010isobutylamiloride (MIA) or bafilomycin A1. This supports our previous conclusion that HCO3\u2010 entry into duct cells from the extracellular fluid requires Na+ but is not dependent on Na(+)\u2010H+ exchange or vacuolar\u2010type H(+)\u2010ATPase activity. 5. The three actions of secretin on guinea\u2010pig pancreatic duct cells described in this and the accompanying paper \u2010 stimulation of a relatively Cl(\u2010)\u2010insensitive luminal HCO3\u2010 efflux pathway, stimulation of basolateral Na(+)\u2010HCO3\u2010 cotransport, and lack of effect on intracellular pH\u2010 require the current model of pancreatic HCO3\u2010 secretion to be modified.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021583","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T07:21:23Z","timestamp":1418800883000},"page":"179-191","source":"Crossref","is-referenced-by-count":107,"title":["Bicarbonate secretion in interlobular ducts from guinea\u2010pig pancreas."],"prefix":"10.1113","volume":"495","author":[{"given":"H","family":"Ishiguro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M C","family":"Steward","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R W","family":"Wilson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R M","family":"Case","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,8,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021583","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021583","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T21:04:39Z","timestamp":1698440679000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021583"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,8,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,8,15]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021583"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021583","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,8,15]]}}}