{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T16:09:47Z","timestamp":1759939787572},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1996,9,1]],"date-time":"1996-09-01T00:00:00Z","timestamp":841536000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,9]]},"abstract":"<jats:p>1. Whole\u2010cell patch\u2010clamp recording techniques were applied to sympathetic preganglionic neurones (SPNs), the majority of which contain neuronal nitric oxide synthase (NOS), in transverse thoracolumbar spinal cord slices from 8\u2010 to 12\u2010day\u2010old rats, and the role of nitric oxide (NO) in modulating the inhibitory postsynaptic current (IPSC) evoked by focal stimulation was examined. 2. Superfusing the slices with the NO precursor L\u2010arginine (L\u2010Arg, 300 microM) and the NO donor sodium nitroprusside (SNP, 100 microM) potentiated IPSCs from several minutes to more than 1 h; the increase was prevented by pretreating the slices with bovine haemoglobin (100 microM), the nitric oxide synthase inhibitor N omega\u2010nor\u2010L\u2010arginine (NO\u2010Arg, 100 microM) or the soluble guanylate cyclase inhibitor 1H\u2010[1,2,4]oxadiazolo[4,3\u2010a]quinoxalin\u20101\u2010one (ODQ, 1 microM). Haemoglobin, NO\u2010Arg and ODQ slightly reduced the IPSCs evoked in some of the SPNs. 3. Superfusion of the slices with N2,2'\u2010O\u2010dibutyrylguanosine 3',5'\u2010cyclic monophosphate (dbcGMP, 300 microM) in the presence of the phosphodiesterase inhibitor 3\u2010isobutyl\u20101\u2010methylxanthine (IBMX, 750 microM) reversibly increased the IPSCs as well. 4. While enhancing the IPSCs, L\u2010Arg or SNP had no significant effects on outward currents induced by pressure application of the putative inhibitory transmitter glycine in the same cells. 5. A train of suprathreshold depolarizing current pulses (30 Hz for 10 s) injected into the recording SPNs caused, after a delay of several minutes, a long\u2010lasting increase of IPSCs; this effect was nullified by superfusing the slices with haemoglobin or NO\u2010Arg. 6. The result suggests that NO released endogenously from SPNs may act as a retrograde messenger molecule to enhance the release of glycine or a related substance, possibly via a cGMP\u2010dependent mechanism.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021608","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T07:26:00Z","timestamp":1418801160000},"page":"479-490","source":"Crossref","is-referenced-by-count":37,"title":["Potentiation of IPSCs by nitric oxide in immature rat sympathetic preganglionic neurones in vitro."],"prefix":"10.1113","volume":"495","author":[{"given":"S Y","family":"Wu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N J","family":"Dun","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,9]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021608","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021608","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T07:26:37Z","timestamp":1698391597000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021608"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,9]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,9]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021608"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021608","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,9]]}}}