{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:44:07Z","timestamp":1758127447326},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1996,10,1]],"date-time":"1996-10-01T00:00:00Z","timestamp":844128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,10]]},"abstract":"<jats:p>1. \u2018Ratiometric\u2019 fura\u20102 methodology in slice preparations and \u2018intensitometric\u2019 fluo\u20103 measurements of confocal images were used to simultaneously monitor Ca2+ mobilization in the two distinct, apically joined cell layers which constitute the ciliary body epithelium (CBE): the non\u2010pigmented (NPE) and pigmented (PE) epithelia. 2. Both methods yielded comparable results regarding Ca2+ responses in the syncytium upon stimulation with adrenergic and cholinergic agonists. 3. The alpha 1\u2010adrenoceptor agonist phenylephrine elicited a moderate [Ca2+]i increase in the PE, whereas NPE [Ca2+]i remained unchanged or exhibited a slight diminution. 4. In combination with carbachol, the alpha 2\u2010adrenoceptor agonist brimonidine elicited large Ca2+ increases (&gt; 10\u2010fold) in both the NPE and PE cell layers, even though previous studies indicated the absence of an alpha 2\u2010adrenergic effect on [Ca2+]i in the PE. The onset, as well as the peak of the Ca2+ responses in PE cells frequently exhibited a small delay with respect to adjacent NPE cells. No such time difference was observed between adjacent NPE cells. 5. Pre\u2010incubation of the ciliary body in Ca(2+)\u2010free solution under conditions known to elicit overt NPE\u2010PE separation abolished the alpha 2\u2010adrenocholinergic response in the PE. 6. Addition of heptanol to the perfusate, to block gap\u2010junctional communication, caused a small [Ca2+]i decrease in the NPE and a slight increase in PE[Ca2+]i. Subsequently, the Ca2+ mobilization in the Pe in response to the brimonidine and carbachol combination was either blocked or showed a substantial delay. The Ca2+ mobilization in the NPE, in contrast, remained unchanged. 7. We conclude that the heterocellular syncytium exhibits rectificatory behaviour with respect to Ca2+ mobilization; responses originating within the NPE are easily transferred to the PE, while the reverse does not occur.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021662","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T07:06:05Z","timestamp":1418799965000},"page":"25-37","source":"Crossref","is-referenced-by-count":18,"title":["Ca2+ mobilization and interlayer signal transfer in the heterocellular bilayered epithelium of the rabbit ciliary body."],"prefix":"10.1113","volume":"496","author":[{"given":"M","family":"Sch\u00fctte","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J M","family":"Wolosin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,10]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021662","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021662","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T16:12:40Z","timestamp":1698336760000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021662"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,10]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,10]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021662"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021662","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,10]]}}}