{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,16]],"date-time":"2026-03-16T10:49:08Z","timestamp":1773658148325,"version":"3.50.1"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1996,10,15]],"date-time":"1996-10-15T00:00:00Z","timestamp":845337600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,10,15]]},"abstract":"<jats:p>1. The effect of synthetic joro spider toxin (JSTX\u20103) on alpha\u2010amino\u20103\u2010hydroxy\u20105\u2010methyl\u20104\u2010isoxazolepropionic acid (AMPA) receptor channels in cultured rat hippocampal neurones was investigated using the whole\u2010cell patch\u2010clamp technique. 2. A population of cultured neurones had AMPA receptors with strong inward rectification and substantial Ca2+ permeability (type II neurones), whereas most neurones (type I neurones) had slight outward rectification and little Ca2+ permeability. JSTX\u20103 selectively suppressed the inwardly rectifying and Ca(2+)\u2010permeable AMPA receptors expressed in type II neurones without affecting AMPA receptors in type I neurones. 3. The effect of JSTX\u20103 on the Ca(2+)\u2010permeable AMPA receptors was use and voltage dependent. In the steady state, current responses induced by ionophoretic applications of kainate (a non\u2010desensitizing agonist of AMPA receptors) were suppressed by the toxin in a dose\u2010dependent manner at negative potentials (IC50 = 56 nM at \u201060 mV). 4. At the standard membrane potential (\u201060 mV), recovery from the blockage by JSTX\u20103 was very slow. Even after washout for more than 7 min, the recovery was only partial. However, the blockage was completely removed immediately after application of a +60 mV voltage pulse for 5 s in conjunction with a single ionophoretic application of kainate.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021696","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T07:11:16Z","timestamp":1418800276000},"page":"431-437","source":"Crossref","is-referenced-by-count":77,"title":["Voltage\u2010dependent blockage of Ca(2+)\u2010permeable AMPA receptors by joro spider toxin in cultured rat hippocampal neurones."],"prefix":"10.1113","volume":"496","author":[{"given":"M","family":"Iino","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M","family":"Koike","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T","family":"Isa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S","family":"Ozawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,10,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021696","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021696","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T19:12:40Z","timestamp":1698347560000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021696"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,10,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1996,10,15]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021696"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021696","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,10,15]]}}}