{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,13]],"date-time":"2025-12-13T09:52:25Z","timestamp":1765619545234},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[1996,11,15]],"date-time":"1996-11-15T00:00:00Z","timestamp":848016000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1996,11,15]]},"abstract":"<jats:p>1. Opioid peptides promote net intestinal absorption via two mechanisms: stimulation of Na+ and Cl\u2010 absorption and inhibition of Cl\u2010 secretion. Although these transport changes are predominantly mediated by submucosal neurones, it is currently unclear whether opioid peptides can regulate enterocyte function directly. We therefore tested the hypothesis that enterocytes have specific opioid receptors. 2. Villus and crypt jejunal epithelial cells were isolated by the distended sac method from anaesthetized guinea\u2010pigs. Flow cytometry was used to resolve enterocytes from other cell types and to determine whether binding of a fluorescently labelled opioid antagonist, naltrexone\u2010FITC, could be prevented by unlabelled mu\u2010 and delta\u2010opioid receptor agonists. A population of crypt enterocytes (approximately 21%) exhibited high\u2010affinity naltrexone\u2010FITC binding to both mu\u2010 and delta\u2010type binding sites that was stereoselective and sodium dependent. Villus enterocytes did not exhibit any of these characteristics. 3. Basal cAMP production was elevated in both villus and crypt cells treated with IBMX (3\u2010isobutyl\u20101\u2010methylxanthine). Villus cells did not respond to 100 nM vasoactive intestinal peptide (VIP), nor were they affected by opioid peptides. In contrast, 100 nM VIP significantly increased cAMP production in crypt epithelial cells, which was significantly reduced by both morphiceptin and D\u2010Ser2\u2010Leu\u2010Enk\u2010Thr. This opioid\u2010mediated effect was stereoselective and blocked by the opioid receptor antagonist naltrexone. 4. These experiments suggest that enterocytes isolated from the crypt epithelium of guineapigs have both mu\u2010 and delta\u2010types of opioid receptors. It is possible that these cells participate in opioid\u2010mediated regulation of intestinal secretion.<\/jats:p>","DOI":"10.1113\/jphysiol.1996.sp021757","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T06:41:38Z","timestamp":1418798498000},"page":"161-174","source":"Crossref","is-referenced-by-count":49,"title":["Opioid receptors on guinea\u2010pig intestinal crypt epithelial cells."],"prefix":"10.1113","volume":"497","author":[{"given":"M E","family":"Lang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J S","family":"Davison","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S L","family":"Bates","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J B","family":"Meddings","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1996,11,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1996.sp021757","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1996.sp021757","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T18:56:40Z","timestamp":1698346600000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1996.sp021757"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,11,15]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[1996,11,15]]}},"alternative-id":["10.1113\/jphysiol.1996.sp021757"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1996.sp021757","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,11,15]]}}}