{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T00:39:50Z","timestamp":1790642390957,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1997,1,15]],"date-time":"1997-01-15T00:00:00Z","timestamp":853286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,1,15]]},"abstract":"<jats:p>1. The effects of cholinergic agonists upon intracellular free Ca2+ levels ([Ca2+]i) have been studied in enzymically isolated rat carotid body single type I cells, using indo\u20101. 2. Acetylcholine (ACh) dose\u2010dependently increased [Ca2+]i in 55% of cells studied (EC50 = 13 microM). These [Ca2+]i rises were partially inhibited by atropine or mecamylamine. 3. Specific nicotinic and muscarinic agonists also elevated [Ca2+]i in a dose\u2010dependent manner (nicotine, EC50 = 15 microM; methacholine, EC50 = 20 microM). 4. While the majority of the ACh\u2010sensitive cells responded to both classes of cholinergic agonist, 29% responded exclusively to nicotinic stimulation and 9% responded exclusively to muscarinic stimulation. 5. In the presence of nicotinic agonists, Ca2+i responses were transient. In the presence of muscarinic agonists, Ca2+i responses consisted of an initial rise, which then declined to a lower plateau level. 6. Nicotinic responses were rapidly abolished in Ca(2+)\u2010free medium, suggesting that they are dependent on Ca2+ influx. 7. The plateau component of the muscarinic\u2010activated response was also abolished in Ca(2+)\u2010free conditions. The rapid initial [Ca2+]i rise, however, could still be evoked after several minutes in Ca(2+)\u2010free medium. Muscarine also increased Mn2+ quenching of intracellular fura\u20102 fluorescence. These data suggest that the full muscarinic response depends on both Ca2+ release from intracellular stores and Ca2+o influx. 8. The results indicate that, in rat carotid body type I cells, both nicotinic and muscarinic acetylcholine receptors increase [Ca2+]i, but achieve this via different mechanisms. ACh may therefore play a role in carotid body function by modulating Ca2+i in the chemosensory type I cells.<\/jats:p>","DOI":"10.1113\/jphysiol.1997.sp021861","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T06:31:43Z","timestamp":1418797903000},"page":"327-338","source":"Crossref","is-referenced-by-count":62,"title":["Muscarinic and nicotinic receptors raise intracellular Ca2+ levels in rat carotid body type I cells."],"prefix":"10.1113","volume":"498","author":[{"given":"L L","family":"Dasso","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K J","family":"Buckler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R D","family":"Vaughan-Jones","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1997,1,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1997.sp021861","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1997.sp021861","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T18:59:29Z","timestamp":1698433169000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1997.sp021861"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,1,15]]}},"alternative-id":["10.1113\/jphysiol.1997.sp021861"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1997.sp021861","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1,15]]}}}