{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T19:58:01Z","timestamp":1762113481928},"reference-count":0,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[1997,1,15]],"date-time":"1997-01-15T00:00:00Z","timestamp":853286400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,1,15]]},"abstract":"<jats:p>1. Whole\u2010cell and single\u2010channel currents elicited by extracellular ATP were studied in freshly dissociated smooth muscle cells from the stomach of the toad Bufo marinus using standard patch clamp and microfluorimetric techniques. 2. This ATP\u2010gated cation channel shares a number of pharmacological and functional properties with native rat myometrium receptors, certain native P2Z purinoceptors and the recently cloned P2X7 purinoceptor. But, unlike the last two, the ATP\u2010gated channel does not mediate the formation of large non\u2010specific pores. Thus, it may represent a novel member of the P2X or P2Z class. 3. Extracellular application of ATP (&gt; or = 150 microM) elicited an inward whole\u2010cell current at negative holding potentials that was inwardly rectifying and showed no sign of desensitization. Na+, Cs+ and, to a lesser degree, the organic cation choline served as charge carriers, but Cl\u2010 did not. Ratiometric fura\u20102 measurements indicated that the current is carried in part by Ca2+. The EC50 for ATP was 700 microM in solutions with a low divalent cation concentration. 4. ATP (&gt; or = 100 microM) at the extracellular surface of cell\u2010attached or excised patches elicited inwardly rectifying single\u2010channel currents with a 22 pS conductance. Cl\u2010 did not serve as a charge carrier but both Na+ and Cs+ did, as did choline to a lesser extent. The mean open time of the channel was quite long, with a range in hundreds of milliseconds at a holding potential of \u201070 mV. 5. Mg2+ and Ca2+ decreased the magnitude of the ATP\u2010induced whole\u2010cell currents. Mg2+ decreased both the amplitude and the activity of ATP\u2010activated single\u2010channel currents. 6. ADP, UTP, P1, P5\u2010di\u2010adenosine pentaphosphate (AP5A), adenosine and alpha, beta\u2010methylene ATP (alpha, beta\u2010Me\u2010ATP) did not induce significant whole\u2010cell current. ATP\u2010gamma\u2010S and 2\u2010methylthio ATP (2\u2010Me\u2010S\u2010ATP) were significantly less effective than ATP in inducing whole\u2010cell currents, whereas benzoylbenzoyl ATP (BzATP) was more effective. BzATP, alpha, beta\u2010Me\u2010ATP, ATP\u2010gamma\u2010S and 2\u2010Me\u2010S\u2010ATP induced single\u2010channel currents, but a higher concentration of alpha, beta\u2010Me\u2010ATP was required. 7. BzATP did not induce the formation of large non\u2010specific pores, as assayed using mag\u2010fura\u20102 as a high molecular mass probe.<\/jats:p>","DOI":"10.1113\/jphysiol.1997.sp021869","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T06:31:43Z","timestamp":1418797903000},"page":"427-442","source":"Crossref","is-referenced-by-count":29,"title":["An ATP\u2010gated cation channel with some P2Z\u2010like characteristics in gastric smooth muscle cells of toad."],"prefix":"10.1113","volume":"498","author":[{"given":"M","family":"Ugur","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R M","family":"Drummond","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H","family":"Zou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P","family":"Sheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J J","family":"Singer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr","given":"J V","family":"Walsh","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[1997,1,15]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1997.sp021869","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1997.sp021869","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T18:59:23Z","timestamp":1698433163000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1997.sp021869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,1,15]]},"references-count":0,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1997,1,15]]}},"alternative-id":["10.1113\/jphysiol.1997.sp021869"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1997.sp021869","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,1,15]]}}}