{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:07:43Z","timestamp":1792570063301,"version":"4.5.0"},"reference-count":0,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[1997,2,1]],"date-time":"1997-02-01T00:00:00Z","timestamp":854755200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Journal of Physiology"],"published-print":{"date-parts":[[1997,2]]},"abstract":"<jats:p>1. Hypoxic stimuli depolarize carotid body type I cells causing voltage\u2010gated calcium influx. This study investigates the cause of this membrane depolarization. Isolated type I cells from neonatal (11\u201016 day) rat carotid bodies were used in the experiments. 2. Tetraethylammonium (TEA; 10 mM), 1 and 5 mM 4\u2010aminopyridine (4\u2010AP) and 20 nM charybdotoxin all failed to evoke a significant rise in [Ca2+]i. Similarly, in perforated patch whole\u2010cell recordings, a combination of 10 mM TEA and 5 mM 4\u2010AP failed to depolarize type I cells. 3. In type I cells voltage clamped at \u201070 mV, anoxia evoked a small inward current under control conditions, but had no effect in the absence of pipette and extracellular K+. 4. Anoxia decreased resting membrane conductance from 322 to 131 pS. The anoxia\u2010sensitive current (measured using voltage ramps from \u2010100 to \u201040 mV) had a reversal potential of \u201089 mV in 4.5 mM Ko+ and \u201066 mV in 20 mM Ko+, indicating that this current was carried principally by potassium ions. In contrast, 10 mM TEA + 5 mM 4\u2010AP had little effect on the current\u2010voltage relationship of the cells over the same range. 5. This O2\u2010sensitive K+ conductance showed only mild outward rectification over the range \u201090 to +30 mV, which could be approximated by the Goldman\u2010Hodgkin\u2010Katz current equation. In addition, there was no time\u2010dependent activation or inactivation of membrane currents elicited by voltage steps in the range \u2010100 to \u201030 mV. 6. The O2\u2010sensitive K+ conductance was inhibited by graded reductions in PO2 to 40 Torr and below, with a K1\/2 of about 12 Torr. 7. The data suggest that hypoxia depolarizes type I cells principally through the inhibition of a small voltage\u2010insensitive resting (or background) K+ conductance, and not through the inhibition of voltage\u2010gated TEA and 4\u2010AP\u2010sensitive K+ channels (e.g. maxi\u2010K or KO2 channels), as has been previously suggested.<\/jats:p>","DOI":"10.1113\/jphysiol.1997.sp021890","type":"journal-article","created":{"date-parts":[[2014,12,17]],"date-time":"2014-12-17T01:36:03Z","timestamp":1418780163000},"page":"649-662","source":"Crossref","is-referenced-by-count":252,"title":["A novel oxygen\u2010sensitive potassium current in rat carotid body type I cells."],"prefix":"10.1113","volume":"498","author":[{"given":"K J","family":"Buckler","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[1997,2]]},"container-title":["The Journal of Physiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1113%2Fjphysiol.1997.sp021890","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/pdf\/10.1113\/jphysiol.1997.sp021890","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,27]],"date-time":"2023-10-27T08:55:23Z","timestamp":1698396923000},"score":1,"resource":{"primary":{"URL":"https:\/\/physoc.onlinelibrary.wiley.com\/doi\/10.1113\/jphysiol.1997.sp021890"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,2]]},"references-count":0,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1997,2]]}},"alternative-id":["10.1113\/jphysiol.1997.sp021890"],"URL":"https:\/\/doi.org\/10.1113\/jphysiol.1997.sp021890","archive":["Portico"],"relation":{},"ISSN":["0022-3751","1469-7793"],"issn-type":[{"value":"0022-3751","type":"print"},{"value":"1469-7793","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,2]]}}}